Differential Recognition of ACE Inhibitors in Xenopus Laevis Oocytes Expressing Rat PEPT1 and PEPT2 (original) (raw)

Abstract

Purpose. To examine the mechanism of inhibition of glycylsarcosine(GlySar) transport by quinapril and enalapril, and whether or notangiotensin converting enzyme (ACE) inhibitors are transported by PEPT2as well as by PEPT1.

Methods. Xenopus laevis oocytes were cRNA-injected with rat PEPT1or PEPT2 and the transport kinetics of radiolabeled GlySar were studiedin the absence and presence of quinapril and enalapril. Thetwo-microelectrode voltage-clamp technique was also performed to probe theelectrogenic uptake of captopril, quinapril and enalapril.

Results. Kinetic analyses demonstrated that quinapril inhibited theuptake of GlySar in a noncompetitive manner in Xenopus oocytesinjected with PEPT1 or PEPT2 (Ki = 0.8 or 0.4 mM, respectively).In contrast, a competitive interaction was observed between GlySarand enalapril (Ki = 10.8 mM for PEPT1 or 4.3 mM for PEPT2).Most significantly, captopril and enalapril, but not quinapril, inducedinwardly-directed currents in both PEPT1- and PEPT2-expressedoocytes.

Conclusions. These results are unique in providing direct evidence forthe substrate recognition and transport of some ACE inhibitors by thehigh- and low-affinity oligopeptide transporters. Our findings point todifferences between PEPT1 and PEPT2 in their affinity to, rather thanin their specificity for, ACE inhibitors.

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Author notes

  1. Angela Steel
    Present address: Anti-Infectives Research, SmithKline Beecham Pharmaceuticals, 1250 S. Collegeville Road, P.O. Box 5089, Collegeville, Pennsylvania, 19426

Authors and Affiliations

  1. College of Pharmacy and Upjohn Center for Clinical Pharmacology, The University of Michigan, Ann Arbor, Michigan, 48109
    Tong Zhu
  2. Membrane Biology Program and Renal Division, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, 02115
    Xing-Zhen Chen, Angela Steel & Matthias A. Hediger
  3. College of Pharmacy and Upjohn Center for Clinical Pharmacology, The University of Michigan, Ann Arbor, Michigan, 48109
    David E. Smith

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  1. Tong Zhu
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  2. Xing-Zhen Chen
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  3. Angela Steel
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  4. Matthias A. Hediger
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  5. David E. Smith
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Zhu, T., Chen, XZ., Steel, A. et al. Differential Recognition of ACE Inhibitors in Xenopus Laevis Oocytes Expressing Rat PEPT1 and PEPT2.Pharm Res 17, 526–532 (2000). https://doi.org/10.1023/A:1007556630189

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