Macrophage Activation by Polymeric Nanoparticles of Polyalkylcyanoacrylates: Activity Against Intracellular Leishmania donovani Associated with Hydrogen Peroxide Production (original) (raw)

Abstract

Nanoparticles of polyalkylcyanoacrylates (PACA) can be useful carrier for the targeting of antileishmanial drugs into macrophages and also possess significant antileishmanial activity by themselves. No significant difference in antileishmanial activity could be detected between nanoparticles of five PACAs with differing alkyl side chains, suggesting that the main degradation products of PACA are not involved in their antileishmanial action. The effect of polyiso-hexylcyanoacrylate (PIHCA) on the induction of the respiratory burst in a macrophage-like cell line (J774G8) was assessed in non-infected macrophages and in macrophages infected with amastigotes of Leishmania donovani infantum, by measuring nitroblue tetrazo-lium (NBT) reduction and hydrogen peroxide production. Phagocytosis of PIHCA nanoparticles led to a respiratory burst, which was more pronounced in infected than in uninfected macrophages. The production of reactive oxygen intermediates associated with the respiratory burst was inhibited by addition of superoxide dismutase and catalase to the cell suspensions. The addition of catalase to the culture medium together with PIHCA nanoparticles significantly reduced the antileishmanial activity of PIHCA. Moreover PIHCA nanoparticles did not induce interleukin-1 release by macrophages. It is suggested that the antileishmanial action of PIHCA and other PACA nanoparticles results from the activation of respiratory burst in macrophages.

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Author notes

  1. Rogério Gaspar
    Present address: Faculty of Pharmacy, Coimbra University, Portugal

Authors and Affiliations

  1. Laboratoire de Pharmacie Galénique, Université Catholique de Louvain, Av. Emmanuel Mounier 73.20, 1200, Brussels, Belgium
    Rogério Gaspar, Veronique Préat & Michel Roland
  2. Research Unit for Tropical Diseases, International Institute of Cellular and Molecular Pathology, Av. Hippocrate 74.39, 1200, Brussels, Belgium
    Fred R. Opperdoes

Authors

  1. Rogério Gaspar
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  2. Veronique Préat
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  3. Fred R. Opperdoes
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  4. Michel Roland
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Gaspar, R., Préat, V., Opperdoes, F.R. et al. Macrophage Activation by Polymeric Nanoparticles of Polyalkylcyanoacrylates: Activity Against Intracellular Leishmania donovani Associated with Hydrogen Peroxide Production.Pharm Res 9, 782–787 (1992). https://doi.org/10.1023/A:1015807706530

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