Mycoplasma contamination of cell cultures: Incidence, sources, effects, detection, elimination, prevention (original) (raw)

• Barile MF and Rottem S (1993) Mycoplasmas in Cell Culture. In: Kahane I and Adoni A (eds) Rapid Diagnosis of Mycoplasmas (pp. 155–193) Plenum Press, New York.
  Google Scholar
• Del Guidice RA and Gardella RS (1984) Mycoplasma Infection of Cell Culture: Effects, Incidence, and Detection. In: Stevenson RE (ed.) Uses and Standardization of Vertebrate Cell Cultures - In Vitro Monograph No. 5 (pp. 104–115) Tissue Culture Association, Gaithersburg, MD.
  Google Scholar
• Drexler HG, Gignac SM, Hu ZB, Hopert A, Fleckenstein E, Voges M and Uphoff CC (1994) Treatment of mycoplasma contamination in a large panel of cell cultures. In Vitro Cell Dev Biol 30A: 344–347.
  CAS Google Scholar
• Drexler HG and Uphoff CC (2000) Contamination of Cell Cultures, Mycoplasma. In: Spier E, Griffiths B and Scragg AH (eds) The Encyclopedia of Cell Technology (pp. 609–627) Wiley, New York.
  Google Scholar
• Drexler HG, Uphoff CC, DirksWG and MacLeod RAF (2002) Mixups and mycoplasma: The enemies within. Leukemia Res 26: 329–333.
  Article Google Scholar
• Fleckenstein E, Uphoff CC and Drexler HG (1994) Effective treatment of mycoplasma contamination in cell lines with enrofloxazin (Baytril). Leukemia 8: 1424–1434.
  PubMed CAS Google Scholar
• Gignac SM, Brauer S, Häne B, Quentmeier H and Drexler HG (1991) Elimination of mycoplasma from infected leukemia cell lines. Leukemia 5: 162–165.
  PubMed CAS Google Scholar
• Gignac SM, Uphoff CC, MacLeod RAF, Steube K, Voges M and Drexler HG (1992) Treatment of mycoplasma-contaminated continuous cell lines with mycoplasma removal agent (MRA). Leukemia Res 16: 815–822.
  Article CAS Google Scholar
• Hay RJ, Macy ML and Chen TR (1989) Mycoplasma infection of cultured cells. Nature 339: 487–488.
  Article PubMed CAS Google Scholar
• Hopert A, Uphoff CC, Wirth M, Hauser H and Drexler HG (1993) Specificity and sensitivity of polymerase chain reaction (PCR) in comparison with other methods for the detection of mycoplasma contamination in cell lines. J Immunol Methods 164: 91–100.
  Article PubMed CAS Google Scholar
• Lo SC, Hayes MM, Tully JG, Wang RYH, Kotani H, Pierce PS, Rose DL and Shih JWK (1992) Mycoplasma penetrans sp. Nov., from the urogenital tract of patients with AIDS. Int J Sys Bact 42: 357–364.
  Article CAS Google Scholar
• McGarrity GJ, Vanaman V and Sarama J (1985) Cell culture techniques. Am Soc Microbiol News 51: 170–183.
  Google Scholar
• McGarrity GJ, Kotani H and Butler GH (1992) Mycoplasmas and Tissue Culture Cells. In: Maniloff J, McElhaney RN, Finch LR and Baseman JB (eds) Mycoplasmas - Molecular Biology and Pathogenesis (pp. 445–454) American Society for Microbiology, Washington, D.C.
  Google Scholar
• Rawadi G and Dussurget O (1995) Advances in PCR-based detection of mycoplasmas contaminating cell cultures. PCR Methods Appl 4: 199–208.
  PubMed CAS Google Scholar
• Razin S and Freundt EA (1984) Mycoplasmatales. In: Holt JG (ed.) Bergey's Manual of Systematic Bacteriology, Vol. 1 (pp. 740–794) Williams and Wilkins, New York.
  Google Scholar
• Razin S (1991) The Genera Mycoplasma, Ureaplasma, Acholeplasma, Anaeroplasma, and Astero-leplasma. In: Balows A, Trümper HG, Dworkin M, Harder W and Schleifer KH (eds) The Prokaryotes, 2nd ed. (pp. 1937–1958) Springer, New York.
  Google Scholar
• Razin S (1994) DNA probes and PCR in diagnosis of mycoplasma infections. Mol Cell Probes 8: 497–511.
  Article PubMed CAS Google Scholar
• Tully JG (1992) Mollicutes (Mycoplasmas). In: Lederberg J (ed.) Encyclopedia of Microbiology (pp. 181–215) Academic Press, San Diego.
  Google Scholar
• Uphoff CC, Gignac SM and Drexler HG (1992) Mycoplasma contamination in human leukemia cell lines. I. Comparison of various detection methods. J Immunol Methods 149: 43–53.
  PubMed CAS Google Scholar
• Uphoff CC, Gignac SM and Drexler HG (1992) Mycoplasma contamination in human leukemia cell lines. II. Elimination with various antibiotics. J Immunol Methods 149: 55–62.
  PubMed CAS Google Scholar
• Uphoff CC, Brauer S, Grunicke D, Gignac SM, MacLeod RAF, Quentmeier H, Steube K, Tümmler M, Voges M, Wagner B and Drexler HG (1992) Sensitivity and specificity of five different mycoplasma detection assays. Leukemia 6: 335–341.
  PubMed CAS Google Scholar
• Uphoff CC and Drexler HG (1999) Detection of mycoplasma contamination in cell cultures by PCR analysis. Human Cell 12: 229–236.
  PubMed CAS Google Scholar
• Uphoff CC and Drexler HG (2001) Prevention of mycoplasma contamination in leukemia-lymphoma cell lines. Human Cell 14: 244–247.
  PubMed CAS Google Scholar
• Uphoff CC and Drexler HG (2002) Comparative PCR analysis for detection of mycoplasma infections in continuous cell lines. In Vitro Cell Dev Biol Anim 38: 79–85.
  Article CAS Google Scholar
• Uphoff CC and Drexler HG (2002) Comparative antibiotic eradication of mycoplasma infections from continuous cell lines. In Vitro Cell Dev Biol Anim 38: 86–89.
  Article CAS Google Scholar
• Uphoff CC and Drexler HG (2002) Detection of mycoplasma in leukemia-lymphoma cell lines using polymerase chain reaction. Leukemia 16: 289–293.
  Article PubMed CAS Google Scholar
• Uphoff CC and Drexler HG (2002) Detecting Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction (PCR). In: Langdon S (ed.) Cancer Cell Culture - Methods and Protocols (in press), Humana Press, Totowa, NJ.
  Google Scholar
• Uphoff CC and Drexler HG (2002) Elimination of Mycoplasma from Infected Cell Lines Using Antibiotics. In: Langdon S (ed.) Cancer Cell Culture -Methods and Protocols (in press), Humana Press, Totowa, NJ.
  Google Scholar
• Uphoff CC, Meyer C and Drexler HG (2002) Elimination of mycoplasma from leukemia-lymphoma cell lines using antibiotics. Leukemia 16: 284–288.
  Article PubMed CAS Google Scholar