Monocyte, macrophage and foreign body giant cell interactions with molecularly engineered surfaces (original) (raw)

Abstract

To elucidate the mechanisms involved in monocyte/macrophage adhesion and fusion to form foreign body giant cells on molecularly engineered surfaces, we have utilized our in vitro culture system to examine surface chemistry effects, cytoskeletal reorganization and adhesive structure development, and cell receptor-ligand interactions in in vitro foreign body giant cell formation. Utilizing silane-modified surfaces, monocyte/macrophage adhesion was essentially unaffected by surface chemistry, however the density of foreign body giant cells (FBGCs) was correlated with surface carbon content. An exception to the surface-independent macrophage adhesion were the alkyl-silane modified surfaces which exhibited reduced adhesion and FBGC formation. Utilizing confocal immunofluorescent techniques, cytoskeletal reorganization and adhesive structure development in in vitro FBGC formation was studied. Podosomes were identified as the adhesive structures in macrophages and FBGCs based on the presence of characteristic cytoplasmic proteins and F-actin at the ventral cell surface. Focal adhesion kinase (FAK) and focal adhesions were not identified as the adhesive structures in macrophages and FBGCs. In studying the effect of preadsorbed proteins on FBGC formation, fibronectin or vitronectin do not play major roles in initial monocyte/macrophage adhesion, whereas polystyrene surfaces modified with RGD exhibited significant FBGC formation. These studies identify the potential importance of surface chemistry-dependent conformational alterations which may occur in proteins adsorbed to surfaces and their potential involvement in receptor-ligand interactions. Significantly, preadsorption of α2-macroglobulin facilitated macrophage fusion and FBGC formation readily on the RGD surface in the absence of any additional serum proteins. As α2-macroglobulin receptors are not found on blood monocytes but are expressed only with macrophage development, these results point to a potential interaction between adsorbed α2-macroglobulin and its receptors on macrophages during macrophage development and fusion. These studies identify important surface independent and dependent effects in foreign body reaction development that may be important in the identification of biological design criteria for molecularly engineered surfaces and tissue engineered devices. © 1999 Kluwer Academic Publishers

Access this article

Log in via an institution

Subscribe and save

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Discover the latest articles, news and stories from top researchers in related subjects.

References

  1. A. K. Mcnally and J. M. Anderson, Am. J. Pathol. 147 (1995) 1487.
    PubMed Google Scholar
  2. K. M. Defife, C. R. Jenney, A. K. Mcnally, E. Colton and J. M. Anderson, J. Immunol. 158 (1997) 3385.
    PubMed Google Scholar
  3. W. J. Kao, A. K. Mcnally, A. Hiltner and J. M. Anderson, J. Biomed. Mater. Res. 29 (1995) 1267.
    PubMed Google Scholar
  4. A. K. Mcnally, K. M. Defife and J. M. Anderson, Am. J. Pathol. 149 (1996) 975.
    PubMed Google Scholar
  5. H. H. Weetal, Appl. Biochem. Biotechnol. 41 (1993) 157.
    PubMed Google Scholar
  6. B. Arkles, ‘Silicon compounds: register and review’, 5th Edn (Hüls America, Inc., Piscataway, NJ, 1991) p. 59.
    Google Scholar
  7. S. R. Wasserman, Y. Tao and G. M. Whitesides, Langmuir 5 (1989) 1074.
    Google Scholar
  8. R. E. Healy, B. Lom and P. E. Hockberger, Biotechnol. Bioengng 43 (1994) 792.
    Google Scholar
  9. G. Truskey and T. Proulx, Biomaterials 14 (1983) 243.
    Google Scholar
  10. D. Kleinfeld, K. Kahler and P. Hockberger, J. Neurosci. 8 (1988) 4098.
    PubMed Google Scholar
  11. S. Margel, E. A. Vogler, L. Firment, T. Watt, S. Haynie and D. Y. Sogah, J. Biomed. Mater. Res. 27 (1993) 1463.
    PubMed Google Scholar
  12. H. L. Yin and J. H. Hartwig, J. Cell Sci. Suppl. 9 (1988) 169.
    PubMed Google Scholar
  13. V. P. Lehto, T. Hovi, T. Vartio, R. A. Badley and I. Virtanen, Lab. Invest. 47 (1982) 391.
    PubMed Google Scholar
  14. P. A. Amato, E. R. Unanue and D. L. Taylor, J. Cell Biol. 96 (1983) 750.
    PubMed Google Scholar
  15. E. P. Reaven and S. G. Axline, ibid. 59 (1973) 12.
    PubMed Google Scholar
  16. J. M. Oliver and R. D. Berlin, in ‘Macrophages and natural killer cells’, edited by S. J. Normann and E. Sorkin (Plenum Press, NY, 1982) p. 113.
    Google Scholar
  17. H. Tapper, J. Leukocyte Biol. 59 (1996) 613.
    PubMed Google Scholar
  18. K. Lewandowska, N. Balachander, C. N. Sukenik and L. A. Culp, J. Cell Physiol. 141 (1989) 334.
    PubMed Google Scholar
  19. L. E. Dike and S. R. Farmer, Proc. Natl Acad. Sci. USA 85 (1988) 6792.
    PubMed Google Scholar
  20. P. C. Marchisio, D. Cirillo, A. Teti, A. Zamboninzallone and G. Tarone, Exp. Cell Res. 169 (1987) 202.
    PubMed Google Scholar
  21. K. Burridge, K. Fath, T. Kelly, G. Nuckolls and C. Turner, Ann. Rev. Cell Biol. 4 (1988) 487.
    PubMed Google Scholar
  22. J. M. Messier, L. M. Shaw, M. Chafel, P. Matsudaira and A. M. Mercurio, Cell Motil. Cytoskel. 25 (1993) 223.
    Google Scholar
  23. K. Burridge, C. E. Turner and L. H. Romer, J. Cell Biol. 119 (1992) 893.
    PubMed Google Scholar
  24. T. H. Lin, A. Yurochko, L. Kornberg, J. Morris, J. J. Walker, S. Haskill and R. L. Juliano, ibid. 126 (1994) 1585.
    PubMed Google Scholar
  25. W. T. Chen, J. Exp. Zool. 251 (1989) 167.
    PubMed Google Scholar
  26. P. M. Henson, Am. J. Pathol. 101 (1980) 494.
    PubMed Google Scholar
  27. S. D. Wright and S. C. Silverstein, Nature 309 (1984) 359.
    PubMed Google Scholar
  28. J. M. Heiple, S. D. Wright, N. S. Allen and S. C. Silverstein, Cell Motil. Cytoskel. 15 (1990) 260.
    Google Scholar
  29. A. Vignery, T. Niven-fairchild, D. H. Ingbar and M. Caplan, J. Histochem. Cytochem. 37 (1989) 1265.
    PubMed Google Scholar
  30. Q. H. Zhao, N. S. Topham, J. M. Anderson, A. Hiltner, G. Lodoen and C. R. Payet, J. Biomed. Mater. Res. 25 (1991) 177.
    PubMed Google Scholar
  31. A. K. Mcnally and J. M. Anderson, Proc. Natl Acad. Sci. USA 91 (1994).
  32. Q. H. Zhao, A. K. Mcnally, K. R. Rubin, M. Renier, Y. Wu, V. Rose-caprara, J. M. Anderson, A. Hiltner, P. Urbanski and K. Stokes, J. Biomed. Mater. Res. 27 (1993) 379.
    PubMed Google Scholar
  33. S. E. Williams, M. Z. Kounnas, K. M. Argraves, W. S. Argraves and D. K. Strickland, Ann. NY Acad. Sci. 737 (1994) 1.
    Google Scholar
  34. L. Sottrup-Jensen, J. Biol. Chem. 264 (1989) 11539.
    PubMed Google Scholar

Download references

Author information

Authors and Affiliations

  1. Institute of Pathology, Department of Macromolecular Science, Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, 44106, USA
    J. M. Anderson, K. Defife & A. Mcnally
  2. Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, 44106, USA
    t. collier & c. jenney

Authors

  1. J. M. Anderson
    You can also search for this author inPubMed Google Scholar
  2. K. Defife
    You can also search for this author inPubMed Google Scholar
  3. A. Mcnally
    You can also search for this author inPubMed Google Scholar
  4. t. collier
    You can also search for this author inPubMed Google Scholar
  5. c. jenney
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

Anderson, J.M., Defife, K., Mcnally, A. et al. Monocyte, macrophage and foreign body giant cell interactions with molecularly engineered surfaces.Journal of Materials Science: Materials in Medicine 10, 579–588 (1999). https://doi.org/10.1023/A:1008976531592

Download citation

Keywords