Bacterial growth based on reductive dechlorination of trichlorobenzenes (original) (raw)

Abstract

An anaerobic mixed bacterial culture was enriched forbacteria dechlorinating 1,2,3- and1,2,4-trichlorobenzene (TCB) to dichlorobenzenes byexclusive use of non-fermentable substrates and theapplication of vancomycin. Growth and dechlorinationoccurred in a purely synthetic medium with formate orhydrogen, acetate, and TCB. Neither acetogenesis normethanogenesis was detected in the culture. Repeatedsubculturing maintaining high dechlorinatingactivities was also achieved when only hydrogen andTCB were supplied. This indicated that reductivedechlorination of TCB was the primary energyconservating process. The number of dechlorinatingbacteria was strictly limited by the amount of TCBsupplied in the medium. In addition, thedechlorinating activity could be maintained only inthe presence of TCB. A most probable number analysisshowed that the dechlorinating species amounted to atleast 6 105 cells per ml at a total cell numberof about 2 106 cells per ml. Vitamin B12significantly stimulated the dechlorinating activity.

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References

• Adrian L, Manz W, Szewzyk U & Görisch H (1998) Physiological characterization of a bacterial consortium reductively dechlorinating 1,2,3-and 1,2,4-trichlorobenzene. Appl. Environ. Microbiol. 64: 496–503
  Google Scholar
• Beurskens JEM, Dekker CGC, van den Heuvel H, Swart M, deWolf J & Dolfing J (1994) Dechlorination of chlorinated benzenes by an anaerobic microbial consortium that selectively mediates the thermodynamic most favorable reactions. Environ. Sci. Technol. 28: 701–706
  Google Scholar
• Bosma TNP, van der Meer JR, Schraa G, Tros ME & Zehnder AJB (1988) Reductive dechlorination of all trichloro-and dichlorobenzene isomers. FEMS. Microbiol. Ecol. 53: 223–229
  Google Scholar
• Christiansen N & Ahring BK (1996) Desulfitobacterium hafniense sp. nov., an anaerobic, reductively dechlorinating bacterium. Int. J. Syst. Bacteriol. 46: 442–448
  Google Scholar
• Cole JR, Cascarelli AL, Mohn WW & Tiedje JM (1994) Isolation and characterization of a novel bacterium growing via reductive dehalogenation of 2-chlorophenol. Appl. Environ. Microbiol. 60: 3536–3542
  Google Scholar
• Dolfing J (1990) Reductive dechlorination of 3-chlorobenzoate is coupled to ATP production and growth in an anaerobic bacterium, strain DCB-1. Arch. Microbiol. 153: 264–266
  Google Scholar
• Dolfing J & Tiedje JM (1987) Growth yield increase linked to reductive dechlorination in a defined 3-chlorobenzoate degrading methanogenic coculture. Arch. Microbiol. 149: 102–105
  Google Scholar
• Fathepure BZ, Tiedje JM & Boyd SA (1988) Reductive dechlorination of hexachlorobenzene to tri-and dichlorobenzenes in anaerobic sewage sludge. Appl. Environ. Microbiol. 54: 327–330
  Google Scholar
• Fathepure BZ & Vogel TM (1991) Complete degradation of polychlorinated hydrocarbons by a two-stage biofilm reactor. Appl. Environ. Microbiol. 57: 3418–3422
  Google Scholar
• Gerritse J, Renard V, Pedro Gomes TM, Lawson PA, Collins MD & Gottschal JC (1996) Desulfitobacterium sp. strain PCE1, an anaerobic bacterium that can grow by reductive dechlorination of tetrachloroethene or ortho-chlorinated phenols. Arch. Microbiol. 165: 132–140
  Google Scholar
• Holliger C, Schraa g, Stams AJM & Zehnder AJB (1992) Enrichment and properties of an anaerobic mixed culture reductively dechlorinating 1,2,3-trichlorobenzene to 1,3-dichlorobenzene. Appl. Environ. Microbiol. 58: 1636–1644
  Google Scholar
• Holliger C, Schraa G, Stams AJM & Zehnder AJB (1993) A highly purified enrichment culture couples the reductive dechlorination of tetrachloroethene to growth. Appl. Environ. Microbiol. 59: 2991–2997
  Google Scholar
• Holliger C, Wohlfarth G & Diekert G (1999) Reductive dechlorination in the energy metabolism of anaerobic bacteria. FEMS Microbiol. Rev. 22: 383–398
  Google Scholar
• Löffler FE, Tiedje JM & Sanford RA (1999) Fraction of electrons consumed in electron acceptor reduction and hydrogen thresholds as indicators of halorespiratory physiology. Appl. Environ. Microbiol. 65: 4049–4056
  Google Scholar
• Mackiewicz M & Wiegel J (1998) Comparison of energy and growth yields for Desulfitobacterium dehalogenans during utilization of chlorophenols and various traditional electron acceptors. Appl. Environ. Microbiol. 64: 352–355
  Google Scholar
• Maymó-Gatell X, Tandoi V, Gossett JM & Zinder SH (1995) Characterization of an H2-utilizing enrichment culture that reductively dechlorinates tetrachloroethene to vinyl chloride and ethene in the absence of methanogenesis and acetogenesis. Appl. Environ. Microbiol. 61: 3928–3933
  Google Scholar
• Maymó-Gatell X, Chien Y-T, Gossett JM & Zinder SH (1997) Isolation of a bacterium that reductively dechlorinates tetrachloroethene to ethene. Science 276: 1568–1571
  Google Scholar
• Middeldorp PJM, de Wolf J, Zehnder AJB & Schraa G (1997) Enrichment and properties of a 1,2,4-trichlorobenzenedechlorinating methanogenic microbial consortium. Appl. Environ. Microbiol. 63: 1225–1229
  Google Scholar
• Mohn WW & Tiedje JM (1990) Strain DCB-1 conserves energy for growth from reductive dechlorination coupled to formate oxidation. Arch. Microbiol. 153: 267–271
  Google Scholar
• Nowak J, Kirsch N-H, Hegemann W & Stan H-J (1996) Total reductive dechlorination of chlorobenzenes to benzene by a methanogenic mixed culture isolated from Saale river sediment. Appl. Microbiol. Biotechnol. 45: 700–709
  Google Scholar
• Pfennig N (1978) Rhodocyclus purpureus gen. nov. and sp. nov., a ring-shaped, vitamin B12-requiring member of the family Rhodospirillaceae. Int. J. Syst. Bacteriol. 28: 283–288
  Google Scholar
• Ramanand K, Balba MT & Duffy J (1993) Reductive dehalogenation of chlorinated benzenes and toluenes under methanogenic conditions. Appl. Environ. Microbiol. 59: 3266–3272
  Google Scholar
• Sanford RA, Cole JR, Löffler FE & Tiedje JM (1996) Characterization of Desulfitobacterium chlororespirans sp. nov., which grows by coupling the oxidation of lactate to the reductive dechlorination of 3-chloro-4-hydroxybenzoate. Appl. Environ. Microbiol. 62: 3800–3808
  Google Scholar
• Scholz-Muramatsu H, Neumann A, Messmer M, Moore E & Diekert G (1995) Isolation and characterization of Dehalospirillum multivorans gen. nov., sp. nov., a tetrachloroethene-utilizing, strictly anaerobic bacterium. Arch. Microbiol. 163: 48–56
  Google Scholar
• Smith PK., Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ & Klenk DC (1985) Measurement of protein using bicinchoninic acid. Anal. Biochem. 150: 76–85
  Google Scholar
• Tsuchiya T & Yamaha T (1984) Reductive dechlorination of 1,2,4-trichlorobenzene by Staphylococcus epidermidis isolated from intestinal contents of rats. Agric. Biol. Chem. 48: 1545–1550
  Google Scholar
• Utkin I, Woese C & Wiegel J (1994) Isolation and characterization of Desulfitobacterium dehalogenans gen. nov., sp. nov., an anaerobic bacterium which reductively dechlorinates chlorophenolic compounds. Int. J. Syst. Bacteriol. 44: 612–619
  Google Scholar
• Zehnder AJB & Wuhrmann K (1976) Titanium(III)citrate as a nontoxic oxidation-reduction buffering system for the culture of obligate anaerobes. Science 194: 1165–1166
  Google Scholar

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Authors and Affiliations

1. Fachgebiet Technische Biochemie, Sekr. GG1, Technische Universität Berlin, Seestraße 13, D-13353, Berlin, Germany
   Lorenz Adrian & Helmut Görisch
2. Fachgebiet Ökologie der Mikroorganismen, Sekr. OE5, Technische Universität Berlin, Franklinstraße 29, D-10587, Berlin, Germany
   Ulrich Szewzyk

Authors

1. Lorenz Adrian
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2. Ulrich Szewzyk
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3. Helmut Görisch
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Adrian, L., Szewzyk, U. & Görisch, H. Bacterial growth based on reductive dechlorination of trichlorobenzenes.Biodegradation 11, 73–81 (2000). https://doi.org/10.1023/A:1026504605443

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• Issue Date: January 2000
• DOI: https://doi.org/10.1023/A:1026504605443