A microfabricated fluorescence-activated cell sorter (original) (raw)

Nature Biotechnology volume 17, pages 1109–1111 (1999)Cite this article

Abstract

We have demonstrated a disposable microfabricated fluorescence-activated cell sorter (μFACS) for sorting various biological entities. Compared with conventional FACS machines, the μFACS provides higher sensitivity, no cross-contamination, and lower cost. We have used μFACS chips to obtain substantial enrichment of micron-sized fluorescent bead populations of differing colors. Furthermore, we have separated Escherichia coli cells expressing green fluorescent protein from a background of nonfluorescent E. coli cells and shown that the bacteria are viable after extraction from the sorting device. These sorters can function as stand-alone devices or as components of an integrated microanalytical chip.

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References

  1. Shapiro, H.M. Practical flow cytometry (Wiley-Liss, New York; 1995 ).
    Google Scholar
  2. Melamed, M.R., Lindmo, T. & Mendelsohn, M.L. Flow cytometry and sorting (Wiley-Liss, New York; 1990).
    Google Scholar
  3. Crosland-Taylor, P.J. A device for counting small particles suspended in a fluid through a tube. Nature 171, 37–38 (1953).
    Article CAS Google Scholar
  4. Coulter, W. Means for counting particles suspended in a fluid. US 2,656,508 (1949).
  5. Kamentsky, L.A., Melamed, M.R. & Derman, H. Spectrometer: new instrument for ultrarapid cell analysis. Science 150, 630–631 (1965).
    Article CAS Google Scholar
  6. Sweet, R.G. High frequency recording with electrostatically deflected ink jets. Rev. Sci. Instrum. 36, 131–133 (1965).
    Article Google Scholar
  7. Fulwyer, M.J. Electronic separation of biological cells by volume. Science 150, 910–911 (1965).
    Article Google Scholar
  8. Brody, J.P. Valveless liquid microswitch. US 656,155 (1998).
  9. Fiedler, S., Shirley, S.G., Schnelle, T. & Fuhr, G. Dielectrophoretic sorting of particles and cells in a microsystem. Anal. Chem. 70, 1909–1915 (1998).
    Article CAS Google Scholar
  10. Li, P.H. & Harrison, D.J. Transport, manipulation, and reaction of biological cells on-chip using electrokinetic effect. Anal. Chem. 69, 1564–1568 (1997).
    Article CAS Google Scholar
  11. Carlson, R.H. et al. Self-sorting of white blood cells in a lattice. Physical Review Letters 79, 2149–2152 (1997).
    Article CAS Google Scholar
  12. Chou, H.P., Spence, C., Scherer, A. & Quake, S.R. A microfabricated device for sizing and sorting DNA. Proc. Natl. Acad. Sci. USA 96, 11–13 (1999).
    Article CAS Google Scholar
  13. Whitesides, G. & Xia, Y. Soft lithography. Angew. Chem. Int. Edn Engl. 37, 550– 575 (1998).
    Article Google Scholar
  14. Kopp, M.U., De Mello, A. & Manz, J.A. Chemical amplification: continuous-flow PCR on a chip. Science 280, 1046–1048 (1998).
    Article CAS Google Scholar
  15. Harrison, D.J. et al. Micromachining a miniaturized capillary electrophoresis-based chemical-analysis system on a chip. Science 261, 895–897 (1993).
    Article CAS Google Scholar
  16. Fodor, S.P.A. et al. Light-directed, spatially addressable parallel chemical synthesis. Science 251, 767–773 (1991).
    Article CAS Google Scholar

Download references

Acknowledgements

This work was supported in part by the Army Research Office and the National Science Foundation. We especially thank Hou-Pu Chou for fabrication of the silicon molds.

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Authors and Affiliations

  1. Department of Applied Physics, California Institute of Technology, Pasadena, 91125, CA
    Anne Y. Fu, Charles Spence, Axel Scherer & Stephen R. Quake
  2. Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, 91125 , CA
    Anne Y. Fu & Frances H. Arnold

Authors

  1. Anne Y. Fu
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  2. Charles Spence
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  3. Axel Scherer
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  4. Frances H. Arnold
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  5. Stephen R. Quake
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Corresponding author

Correspondence toStephen R. Quake.

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Fu, A., Spence, C., Scherer, A. et al. A microfabricated fluorescence-activated cell sorter.Nat Biotechnol 17, 1109–1111 (1999). https://doi.org/10.1038/15095

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