Detection of Sugars on Paper Chromatograms (original) (raw)

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• Published: 09 September 1950

Nature volume 166, pages 444–445 (1950)Cite this article

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Abstract

THE ammoniacal silver nitrate spray1 used for the detection of sugars has several disadvantages; to those mentioned by Partridge2 should be added the necessity for very careful control of the heating step, particularly important in laboratories lacking special apparatus. We have introduced modifications, based on a test given by Feigl3 for reducing sugars, which eliminate the heating step, and in which the reagents are applied in organic solvents, thus removing the danger of migration of the sugar spots. The method has been in use for more than a year, and has proved easy to handle and extremely reliable.

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References

1. Partridge, S. M., Biochem. J., 42, 238 (1948).
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2. Partridge, S. M., Nature, 164, 443 (1949).
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3. Feigl, F., “_Quantitative Analysis by Spot Tests_”, 3rd English edit. (New York–Amsterdam: Elsevier, 1947.)
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4. Seidell, A., “_Solubilities of Organic Compounds_”, 3rd edit. (New York: Van Nostrand co., 1941.)
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5. Hough, L., Nature, 165, 400 (1950).
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6. Mattson, A. M., and Jensen, C. O., Anal. Chem., 22, 182 (1950).
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Authors and Affiliations

1. Research and Development Department, Distillers Company, Limited, Epsom, Surrey
   W. E. TREVELYAN, D. P. PROCTER & J. S. HARRISON

Authors

1. W. E. TREVELYAN
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2. D. P. PROCTER
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3. J. S. HARRISON
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TREVELYAN, W., PROCTER, D. & HARRISON, J. Detection of Sugars on Paper Chromatograms.Nature 166, 444–445 (1950). https://doi.org/10.1038/166444b0

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• Issue Date: 09 September 1950
• DOI: https://doi.org/10.1038/166444b0

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