Gene silencing in Neurospora crassa requires a protein homologous to RNA-dependent RNA polymerase (original) (raw)

References

  1. Flavell, R. B. Inactivation of gene expression in plants as a consequence of specific sequence duplication. Proc. Natl Acad. Sci. USA 91, 3490–3496 (1994).
    Article ADS CAS Google Scholar
  2. Depicker, A. & Van Montagu, M. Post-transcriptional gene silencing in plants. Curr. Opin. Cell Biol. 9, 373–382 (1997).
    Article Google Scholar
  3. Ratcliff, F., Harrison, B. D. & Baulcombe, D. C. Asimilarity between viral defense and gene silencing in plants. Science 276, 1558–1560 (1997).
    Article CAS Google Scholar
  4. Voinnet, O. & Baulcombe, D. C. Systemic signalling in gene silencing. Nature 389, 553 (1997).
    Article ADS CAS Google Scholar
  5. Kasschau, K. D. & Carrington, J. C. Acounterdefensive strategy of plant viruses: suppression of posttranscriptional silencing. Cell 95, 461–470 (1998).
    Article CAS Google Scholar
  6. Assaad, F. F., Lee Tucker, K. & Signer, E. R. Epigenetic repeat-induced gene silencing (RIGS) in Arabidopsis. Plant Mol. Biol. 22, 1067–1085 (1993).
    Article CAS Google Scholar
  7. Bingham, P. M. Cosuppression comes to the animals. Cell 90, 385–387 (1997).
    Article CAS Google Scholar
  8. Cogoni, C. & Macino, G. Isolation of quelling-defective (qde) mutants impaired in posttranscriptional transgene-induced gene silencing in Neurospora crassa. Proc. Natl Acad. Sci. USA 94, 10233–10238 (1997).
    Article ADS CAS Google Scholar
  9. Schiebel, W. et al. Isolation of an RNA-directed RNA polymerase-specific cDNA clone from tomato. Plant Cell 10, 2087–2101 (1998).
    CAS PubMed PubMed Central Google Scholar
  10. Palauqui, J. C., Elmayan, T., Pollien, J. M. & Vaucheret, H. Systemic acquired silencing: transgene-specific post-transcriptional silencing is transmitted by grafting from silenced stocks to non-silenced scions. EMBO J. 16, 4738–4745 (1997).
    Article CAS Google Scholar
  11. Cogoni, C. et al. Transgene silencing of the al-1 gene in vegetative cells of Neurospora is mediated by a cytoplasmic effector and does not depend on DNA–DNA interactions or DNA methylation. EMBO J. 15, 3153–3163 (1996).
    Article CAS Google Scholar
  12. Stam, M., Mol, J. N. M. & Kooter, J. M. The silence of genes in transgenic plants. Ann. Bot. 79, 3–12 (1997).
    Article CAS Google Scholar
  13. Voinnet, O., Vain, P., Angell, S. & Baulcombe, D. C. Systemic spread of sequence-specific transgene RNA degradation in plants is initiated by localized introduction of ectopic promoterless DNA. Cell 95, 177–187 (1998).
    Article CAS Google Scholar
  14. Metzlaff, M., O'Dell, M., Cluster, P. D. & Flavell, R. B. RNA-mediated RNA degradation and chalcone synthase A silencing in Petunia. Cell 88, 845–854 (1997).
    Article CAS Google Scholar
  15. Wassenegger, M. & Pélissier, T. Amodel for RNA-mediated gene silencing in higher plants. Plant Mol. Biol. 37, 349–362 (1998).
    Article CAS Google Scholar
  16. Baulcombe, D. C. RNA as a target and an initiator of post-transcriptional gene silencing in transgenic plants. Plant Mol. Biol. 32, 79–88 (1996).
    Article CAS Google Scholar
  17. Romano, N. & Macino, G. Quelling: transient inactivation of gene expression in Neurospora crassa by transformation with homologous sequences. Mol. Microbiol. 6, 3343–3353 (1992).
    Article CAS Google Scholar
  18. Cogoni, C. & Macino, G. Conservation of transgene-induced post-transcriptional gene silencing in plants and fungi. Trends Plant Sci. 2, 438–443 (1997).
    Article Google Scholar
  19. Schmidhauser, T. J., Lauter, F. R., Russo, V. E. A. & Yanofsky, C. Cloning, sequence, and photoregulation of al-1, a carotenoid biosynthetic gene of Neurospora crassa. Mol. Cell Biol. 10, 5064–5070 (1990).
    Article CAS Google Scholar
  20. Lindbo, J. A., Silva-Rosales, L., Proebsting, W. M. & Dougherty, W. G. Induction of a highly specific antiviral state in transgenic plants: implications for regulation of gene expression and virus resistance. Plant Cell 5, 1749–1759 (1993).
    Article CAS Google Scholar
  21. Montgomery, M. K. & Fire, A. Double-stranded RNA as mediator in sequence-specific genetic silencing and co-suppression. Trends Genet. 14, 255–258 (1998).
    Article CAS Google Scholar
  22. Fire, A. et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391, 806–811 (1998).
    Article ADS CAS Google Scholar
  23. Timmons, L. & Fire, A. Specific interference by ingested dsRNA. Nature 395, 854 (1998).
    Article ADS CAS Google Scholar
  24. Matzke, M. A., Matzke, A. J. M. & Eggleston, W. B. Paramutation and transgene silencing: a common response to invasive DNA? Trends Plant Sci. 1, 382–388 (1996).
    Article Google Scholar
  25. Davis, R. H. & DeSerres, F. J. Genetic and microbiological research techniques for Neurospora crassa. Methods Enzymol. 17, 79–143 (1970).
    Article Google Scholar
  26. Vollmer, L. J. & Yanofsky, C. Efficient cloning of genes of Neurospora crassa. Proc. Natl Acad. Sci. USA 83, 4869–4873 (1986).
    Article ADS CAS Google Scholar
  27. Staben, C. et al. Use of a bacterial Hygromycin B resistance gene as a dominant marker in Neurospora crassa transformation. Fungal Genet. Newslett. 36, 79–81 (1989).
    Google Scholar
  28. Cabibbo, A., Sporeno, E., Macino, G. & Ballario, P. CBM1, an Neurospora crassa genomic library in pAC3 and its use for walking on chromosome VII right arm. Fungal Genet. Newslett. 38, 68–70 (1991).
    Google Scholar
  29. Morelli, G., Nelson, M. A., Ballario, P. & Macino, G. Photoregulated carotenoid biosynthetic genes of Neurospora crassa. Methods Enzymol. 214, 412–424 (1993).
    Article CAS Google Scholar
  30. Maniatis, S. T., Fritsch, E. F. & Sambrook, J. Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press, New York, (1982).
    Google Scholar

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