Structure of the nuclear transport complex karyopherin-β2–Ran˙GppNHp (original) (raw)

References

  1. Pemberton, L. F., Blobel, G. & Rosenblum, J. S. Transport routes through the nuclear pore complex. Curr. Opin. Cell Biol. 10, 392– 399 (1998).
    Article CAS Google Scholar
  2. Nehrbass, U. & Blobel, G. Role of the nuclear transport factor p10 in nuclear import. Science 272, 120– 122 (1996).
    Article ADS CAS Google Scholar
  3. Ribbeck, K., Lipowsky, G., Kent, H. M., Stewart, M. & Gorlich, D. NTF2 mediates nuclear import of Ran. EMBO J. 17, 6587–6598 (1998).
    Article CAS Google Scholar
  4. Conti, E., Uy, M., Leighton, L., Blobel, G. & Kuriyan, J. Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin alpha. Cell 94, 193–204 ( 1998).
    Article CAS Google Scholar
  5. Albertini, M., Pemberton, L. F., Rosenblum, J. S. & Blobel, G. Anovel nuclear import pathway for the transcription factor TFIIS. J. Cell Biol. 143, 1447–1455 (1998).
    Article CAS Google Scholar
  6. Kaffman, A., Rank, N. M. & O'Shea, E. K. Phosphorylation regulates association of the transcription factor Pho4 with its import receptor Pse1/Kap121. Genes Dev. 12, 2673–2683 (1998).
    Article CAS Google Scholar
  7. Kaffman, A., Rank, N. M., O'Neill, E. M., Huang, L. S. & O'Shea, E. K. The receptor MsN5 exports the phosphorylated transcription factor Pho4 out of the nucleus. Nature 396, 482–486 ( 1998).
    Article ADS CAS Google Scholar
  8. Rexach, M. & Blobel, G. Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins. Cell 83, 683– 692 (1995).
    Article CAS Google Scholar
  9. Siomi, M. C. et al. Transportin-mediated nuclear import of heterogeneous nuclear RNP proteins. J.Cell Biol. 138, 1181– 1192 (1997).
    Article CAS Google Scholar
  10. Izaurralde, E., Kutay, U., von Kobbe, C., Mattaj, I. W. & Gorlich, D. The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus. EMBO J. 16, 6535–6547 ( 1997).
    Article CAS Google Scholar
  11. Bischoff, F. R. & Ponstingl, H. Catalysis of guanine nucleotide exchange of Ran by RCC1 and stimulation of hydrolysis of Ran-bound GTP by Ran-GAP1. Methods Enzymol. 257, 135–144 (1995).
    Article CAS Google Scholar
  12. Bonifaci, N., Moroianu, J., Radu, A. & Blobel, G. Karyopherin beta2 mediates nuclear import of a mRNA binding protein. Proc. Natl Acad. Sci. USA 94, 5055–5060 ( 1997).
    Article ADS CAS Google Scholar
  13. Pollard, V. W. et al. Anovel receptor-mediated nuclear protein import pathway. Cell 86, 985–994 ( 1996).
    Article CAS Google Scholar
  14. Fridell, R. A., Truant, R., Thorne, L., Benson, R. E. & Cullen, B. R. Nuclear import of hnRNP A1 is mediated by a novel cellular cofactor related to karyopherin-beta. J. Cell Sci. 110, 1325–1331 (1997).
    CAS PubMed Google Scholar
  15. Andrade, M. A. & Bork, P. HEAT repeats in Huntington's disease protein. Nature Genet. 11, 115– 116 (1995).
    Article CAS Google Scholar
  16. Stewart, M., Kent, H. M. & McCoy, A. J. The structure of the Q69L mutant of GDP-Ran shows a major conformational change in the switchII loop that accounts for its failure to bind nuclear transport factor2 (NTF2). J. Mol. Biol. 284, 1517–1527 (1998).
    Article CAS Google Scholar
  17. Scheffzek, K., Klebe, C., Fritz-Wolf, K., Kabsch, W. & Wittinghofer, A. Crystal structure of the nuclear Ras-related protein Ran in its GDP-bound form. Nature 374, 378–381 (1995).
    Article ADS CAS Google Scholar
  18. Sheldrick, G. M., Dauter, Z., Wilson, K. S., Hope, H. & Sieker, L. C. The application of direct methods and Patterson interpretation to high-resolution native protein data. Acta Crystallogr. D 49, 18–23 (1993).
    Article CAS Google Scholar
  19. Groves, M. R., Hanlon, N., Turowski, P., Hemmings, B. A. & Barford, D. The structure of the protein phosphatase 2A PR65/A subunit reveals the conformation of its 15 randomly repeated HEAT motifs. Cell 96, 99–110 ( 1999).
    Article CAS Google Scholar
  20. Huber, A. H., Nelson, W. J. & Weis, W. I. Three-dimensional structure of the armadillo repeat region of β-catenin. Cell 90, 871– 882 (1997).
    Article CAS Google Scholar
  21. Malik, H. S., Eickbush, T. H. & Goldfarb, D. S. Evolutionary specialization of the nuclear targeting apparatus. Proc. Natl Acad. Sci. USA 94, 13738–3742 (1997).
    Article ADS CAS Google Scholar
  22. Richards, S. A., Lounsbury, K. M. & Macara, I. G. The C terminus of the nuclear RAN/TC4 GTPase stabilizes the GDP-bound state and mediates interactions with RCC1, RAN-GAP, and HTF9A/RANBP1. J. Biol. Chem. 270, 14405– 14411 (1995).
    Article CAS Google Scholar
  23. Vetter, I. R., Nowak, C., Nishimoto, T., Kuhlmann, J. & Wittinghofer, A. Structure of a Ran-binding domain complexed with Ran bound to a GTP analogue: implications for nuclear transport. Nature 398, 39–46 ( 1999).
    Article ADS CAS Google Scholar
  24. Pai, E. F. et al. Structure of the guanine-nucleotide-binding domain of the Ha- ras oncogene product p21 in the triphosphate conformation. Nature 341, 209–214 ( 1989).
    Article ADS CAS Google Scholar
  25. Hidea, M. et al. Amonoclonal antibody to the COOH-terminal acidic portion of Ran inhibits both the recycling of Ran and nuclear protein import in living cells. J. Cell Biol. 144, 645– 655 (1999).
    Article Google Scholar
  26. Kutay, U., Izaurralde, E., Bischoff, F. R., Mattaj, I. W. & Gorlich, D. Dominant-negative mutants of important-beta block multiple pathways of import and export through the nuclear pore complex. EMBO J. 16, 1153–1163 (1997).
    Article CAS Google Scholar
  27. Kose, S., Imamoto, N., Tachibana, T., Shimamoto, T. & Yoneda, Y. Ran-unassisted nuclear migration of a 97-kD component of nuclear pore-targeting complex. J. Cell Biol. 139, 841–849 ( 1997).
    Article CAS Google Scholar
  28. Chi, N. C., Adam, E. J. H. & Adam, S. A. Different binding domains for Ran-GTP and Ran-GDP/RanBP1 on nuclear import factor p97. J. Biol. Chem. 272, 6818–6822 (1997).
    Article CAS Google Scholar
  29. Moroianu, J., Blobel, G. & Radu, A. Nuclear protein import: Ran-GTP dissociates the karyopherin alphabeta heterodimer by displacing alpha from an overlapping binding site on beta. Proc. Natl Acad. Sci. USA 93, 7059 –7062 (1996).
    Article ADS CAS Google Scholar
  30. Enenkel, C., Schulke, N. & Blobel, G. Expression in yeast of binding regions of karyopherins alpha and beta inhibits nuclear import and cell growth. Proc. Natl Acad. Sci. USA 93, 12986–12991 (1996).
    Article ADS CAS Google Scholar
  31. Gorlich, D., Pante, N., Kutay, U., Aebi, U. & Bischoff, F. R. Identification of different roles for RanGDP and RanGTP in nuclear protein import. EMBO J. 15, 5584–5594 (1996).
    Article CAS Google Scholar
  32. Floer, M. & Blobel, G. The nuclear transport factor karyopherin beta binds stoichiometrically to Ran-GTP and inhibits the Ran GTPase activating protein. J. Biol. Chem. 271, 5313– 5316 (1996).
    Article CAS Google Scholar
  33. Lounsbury, K. M. & Macara, I. G. Ran-binding protein 1 (RanBP1) forms a ternary complex with Ran and karyopherin beta and reduces Ran GTPase-activating protein (RanGAP) inhibition by karyopherin beta. J. Biol. Chem. 272, 551– 555 (1997).
    Article CAS Google Scholar
  34. Scheffzek, K. et al. The Ras-RasGAP complex: Structural basis for GTPase activation and its loss in oncogenic Ras mutants. Science 277, 333–338 (1997).
    Article CAS Google Scholar
  35. Rittinger, K., Walker, P. A., Eccleston, J. F., Smerdon, S. J. & Gamblin, S. J. Structure at 1.65 Å of RhoA and its GTPase-activating protein in complex with a transition-state analogue. Nature 389, 758– 762 (1997).
    Article ADS CAS Google Scholar
  36. Boriack-Sjodin, P. A., Margarit, S. M., Bar-Sagi, D. & Kuriyan, K. The structural basis of the activation of Ras by Sos. Nature 394, 337–343 (1998).
    Article ADS CAS Google Scholar
  37. Coutavas, E., Ren, M., Oppenheim, J. D., E'Eustachio, P. & Rush, M. G. Characterization of proteins that interact with the cell-cycle regulatory protein Ran/TC4. Nature 366, 585–587 ( 1993).
    Article ADS CAS Google Scholar
  38. Wu, J., Matunis, M. J., Kraemer, D., Blobel, G. & Coutavas, E. Nup358, a cytoplasmically exposed nucleoporin with peptide repeats, Ran-GTP binding sites, zinc fingers, a cyclophilin A homologous domain, and a leucine-rich region. J. Biol. Chem. 270, 14209–14213 ( 1995).
    Article CAS Google Scholar
  39. Yokoyama, N. et al. Agiant nucleopore protein that binds Ran/TC4. Nature 376, 184–188 ( 1995).
    Article ADS CAS Google Scholar
  40. Bischoff, F. R. & Gorlich, D. RanBP1 is crucial for the release of RanGTP from importin beta-related nuclear transport factors. FEBS Lett. 419, 249–254 (1997).
    Article CAS Google Scholar
  41. Kuhlmann, J., Macara, I. & Wittinghofer, A. Dynamic and equilibrium studies on the interaction of Ran with its effector, RanBP1. Biochemistry 36, 12027–12035 (1997).
    Article CAS Google Scholar
  42. Van Duyne, G. D., Standaert, R. F., Karplus, P. A., Schreiber, S. L. & Clardy, J. Atomic structure of FKBP-FK506, an immunophilin-immunosuppressant complex. Science 251, 839–842 ( 1991).
    Article ADS Google Scholar
  43. Westbrook, E. M. & Naday, I. Charge-coupled device-based area detectors. Methods Enzymol. 276, 244 –268 (1997).
    Article CAS Google Scholar
  44. Otwinowski, Z. & Minor, W. Processing of X-ray diffraction data collected in oscillation model. Methods Enzymol. 276, 307–326 ( 1997).
    Article CAS Google Scholar
  45. CCP4. The CCP4 suite: programs for X-ray crystallography. Acta Crystallogr. D 50, 760– 776 (1994).
  46. Jones, T. A., Cowan, S. W. & Kjelgaard, M. Improved methods for building protein models in electron density maps and the location of errors in these models. Acta Crystallogr. A 47, 110–119 ( 1991).
    Article Google Scholar
  47. Brunger, A. T. et al. Crystallography & NMR system: A new software suite for macromolecular structure determination. Acta Crystallogr. D 54, 905–921 (1998).
    Article CAS Google Scholar
  48. Kraulis, P. MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures. J. Appl. Crystallogr. 24, 946 –950 (1991).
    Article Google Scholar
  49. Merritt, E. A. & Murphy, M. E. P. Raster3D Version 2.0. A program for photorealistic molecular graphics. Acta Crystallog. D 50, 869–873 (1994).
    Article CAS Google Scholar
  50. Nicholls, A., Sharp, K. A. & Honig, B. Protein folding and association: insights from the interfacial and thermodynamic properties of hydrocarbons. Proteins Struct. Funct. Genet. 11, 281–296 (1991).
    Article CAS Google Scholar

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