Application of Particle-counting to a Leafhopper-borne Virus (original) (raw)

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• Published: 08 July 1967

Nature volume 215, pages 173–174 (1967)Cite this article

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Abstract

THE electron microscope particle-counting technique of Backus and Williams1,2, which involves the use of shadow-cast specimens, has been generally favoured in investigations of viruses involving particle counting. Watson3 modified the method by using phosphotungstate negative staining4,5 to reveal the virus particles in more structural detail and to make identification easier. The modification facilitates counting of virus particles in relatively impure preparations.

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References

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Authors and Affiliations

1. Department of Botany, University of Illinois, Urbana, Illinois
   R. GÁMEZ & L. M. BLACK
2. Departamento de Fitopatología, Universidad de Costa Rica, Ciudad Universitaria, Costa Rica
   R. GÁMEZ

Authors

1. R. GÁMEZ
2. L. M. BLACK

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GÁMEZ, R., BLACK, L. Application of Particle-counting to a Leafhopper-borne Virus.Nature 215, 173–174 (1967). https://doi.org/10.1038/215173a0

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• Received: 23 February 1967
• Revised: 04 April 1967
• Issue date: 08 July 1967
• DOI: https://doi.org/10.1038/215173a0

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