Lac Repressor Binding to DNA not containing the Lac Operator and to Synthetic Poly dAT (original) (raw)

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• Published: 19 December 1970

Nature volume 228, pages 1184–1186 (1970)Cite this article

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Abstract

THE lac represser binds very specifically and tightly to the lac operator1–3. In conditions where the repressor binds to operator with a dissociation constant of 10−13 M, the binding of represser to DNA not containing the Escherichia coli lac operator (non-operator DNA) was less tight by several orders of magnitude3. Although weak, we thought this non-operator DNA binding could be highly significant and deserved careful study. We report here on the binding of lac represser to non-operator DNA from several natural sources and also to synthetic poly dAT.

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Authors and Affiliations

1. Division of Biology, City of Hope National Medical Center, Duarte, California, 91010
   SYR-YAUNG LIN & ARTHUR D. RIGGS

Authors

1. SYR-YAUNG LIN
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2. ARTHUR D. RIGGS
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LIN, SY., RIGGS, A. Lac Repressor Binding to DNA not containing the Lac Operator and to Synthetic Poly dAT.Nature 228, 1184–1186 (1970). https://doi.org/10.1038/2281184a0

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• Received: 04 September 1970
• Revised: 10 October 1970
• Issue Date: 19 December 1970
• DOI: https://doi.org/10.1038/2281184a0

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