Isolation of a collagen-dependent cell attachment factor (original) (raw)

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• Published: 19 July 1974

Nature volume 250, pages 248–251 (1974)Cite this article

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Abstract

MOST untransformed mammalian cells require an appropriate surface for survival and growth in vitro; this phenomenon has been termed anchorage dependence1. Glass, tissue culture plastics, fibrin clots and collagen surfaces have long been recognised as substrates able to support the attachment and growth of cells. Since the cell plasma membrane is separated from plastics substrates by a 450 Å layer of electron opaque material2, the nature of the 450 Å would appear to be more important in cell attachment than the chemical composition of the plastic. It is demonstrated here that cell attachment to collagen is mediated by a high molecular weight protein present in serum.

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Author notes

1. ROBERT J. KLEBE
   Present address: Division of Human Genetics, Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch, Galveston, Texas, 77550

Authors and Affiliations

1. The Salk Institute, San Diego, California, 92112
   ROBERT J. KLEBE

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KLEBE, R. Isolation of a collagen-dependent cell attachment factor.Nature 250, 248–251 (1974). https://doi.org/10.1038/250248a0

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• Received: 01 October 1973
• Revised: 25 February 1974
• Issue date: 19 July 1974
• DOI: https://doi.org/10.1038/250248a0

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