Functional fractionation of human cytotoxic cells using differences in their cation requirements (original) (raw)
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- Published: 05 June 1975
Nature volume 255, pages 491–493 (1975)Cite this article
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Abstract
DEMONSTRATION of anti-tumour immunity in man is often impaired by the variable level of cytoxicity of normal control cell populations. Using physical fractionation techniques, attempts have been made to remove selectively the cells which are responsible for this ‘variable background’ effect1,2, and to improve characterisation of cytotoxic cell populations. We report here that several types of human cell-mediated cytolysis have different extracellular cation requirements either for Ca2+, for Mg2+, for both, or for neither. These findings suggest the existence of differences in the mechanism(s) of lysis, and lead us to propose an alternative, functional approach to the fractionation of cytotoxic cells.
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Authors and Affiliations
- Tumour Immunology Unit, Department of Zoology, University College London, Gower Street, London, WC1E 6BT, UK
PIERRE GOLSTEIN - Department of Experimental Pathology, University College Hospital Medical School, Gower Street, London, WC1E 6JJ, UK
CLARE FEWTRELL
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- PIERRE GOLSTEIN
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GOLSTEIN, P., FEWTRELL, C. Functional fractionation of human cytotoxic cells using differences in their cation requirements.Nature 255, 491–493 (1975). https://doi.org/10.1038/255491a0
- Received: 04 March 1975
- Accepted: 21 April 1975
- Issue Date: 05 June 1975
- DOI: https://doi.org/10.1038/255491a0