Nitrogenase activity in rhizobia in absence of plant host (original) (raw)

Nature volume 256, pages 407–409 (1975) Cite this article

Abstract

RHIZOBIA can infect legume roots and fix dinitrogen in the root nodules. Repeated attempts have failed to demonstrate fixation by rhizobia on defined medium or in plant extracts. Rhizobium japonicum grown anaerobically with nitrate produces a haemoprotein pattern similar to that of N2-fixing bacteroids1 and synthesises the Mo–Fe protein of nitrogenase2, but it has seemed that infection of legume cells was a prerequisite for the expression of nitrogenase. It has been shown recently3,4, however, that cowpea Rhizobium 32H1 could fix dinitrogen when grown close to, but not in contact with, callus cultures of legumes or non-legumes. This supported other evidence5,6 that it was the rhizobia which contained the genetic information for nitrogenase, and showed that its expression was promoted by diffusable factors from plant cells.

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References

  1. Daniel, R. M., and Appleby, C. A., Biochim. biophys. Acta, 275, 347–354 (1972).
    Article CAS Google Scholar
  2. Bishop, P. E., Evans, H. J., Daniel, R. M., and Hampton, R. O., Biochim. biophys. Acta, 381, 248–256 (1975).
    Article CAS Google Scholar
  3. Child, J. J., Nature, 253, 350–351 (1975).
    Article ADS CAS Google Scholar
  4. Scowcroft, W. R., and Gibson, A. H., Nature, 253, 351–352 (1975).
    Article ADS CAS Google Scholar
  5. Dunican, L. K., and Tierney, A. B., Biochem. biophys. Res. Commun., 57, 62–72 (1973).
    Article Google Scholar
  6. Phillips, D. A., Howard, R. L., and Evans, H. J., Physiol. Pl., 28, 248–253 (1973).
    Article CAS Google Scholar
  7. Child, J. J., and LaRue, T. A., Pl. Physiol., Lancaster, 53, 88–90 (1974).
    Article CAS Google Scholar
  8. Hugh, R., and Leifson, E., J. Bact., 6, 24–26 (1953).
    Google Scholar
  9. Gordon, J. K., and Brill, W. J., Biochem. biophys. Res. Commun., 59, 967–971 (1974).
    Article CAS Google Scholar
  10. Wilson, P. W., in Encyclopaedia of Plant Physiology, VIII, (edit. by Ruhland, W.), (Springer, Berlin, 1958).
    Google Scholar
  11. Hill, S., Drozd, J. W., and Postgate, J. R., J. appl. Chem. Biotechnol., 22, 541–552 (1972).
    Article CAS Google Scholar
  12. Postgate, J. R., The Chemistry and Biochemistry of Nitrogen Fixation (Plenum, London, 1971).
    Google Scholar

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Authors and Affiliations

  1. National Research Council of Canada, Prairie Regional Laboratory, Saskatoon, Saskatchewan, S7N OW9, Canada
    W. G. W. KURZ & T. A. LARUE

Authors

  1. W. G. W. KURZ
  2. T. A. LARUE

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KURZ, W., LARUE, T. Nitrogenase activity in rhizobia in absence of plant host.Nature 256, 407–409 (1975). https://doi.org/10.1038/256407a0

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