Binding sites related to ouabain-induced stimulation or inhibition of the sodium pump (original) (raw)

Nature volume 265, pages 165–166 (1977)Cite this article

Abstract

THE concentration of free cardiac glycosides in the blood of patients treated for heart failure ranges between 1 and 5 nM (refs 1–3). Studies on human heart slices have shown that, within this range of concentrations, digitoxin does not inhibit the sodium pump but does stimulate 42K uptake3. Similar observations have been made with isolated guinea pig atria: low concentrations of ouabain (near 1 nM) stimulate 42K uptake whereas higher concentrations inhibit it4. These results are in agreement with recent electrophysiological observations on Purkinje fibres showing that low doses of ouabain produce changes in the K gradient that reflect stimulation of the sodium pump5. It has also been observed that the stimulation of the sodium pump by ouabain is associated with a positive ionotropic effect6. Earlier studies with guinea pig atria4 and other preparations7–9 showed that the binding of ouabain occurs on non-specific and specific sites. The specific binding is fitted by a Langmuir curve with an equilibrium constant close to that estimated by measuring the inhibition of the sodium pump. We report here the existence of a binding with a higher affinity associated with the stimulation of the sodium pump.

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References

  1. Grahame-Smith, D. G., and Everest, M. S., Br. med. J., 1, 286–289 (1969).
    Article CAS Google Scholar
  2. Lukas, D. S., Ann. N.Y. Acad. Sci., 179, 338–361 (1971).
    Article ADS CAS Google Scholar
  3. Godfraind, T., Archs int. Pharmacodyn. Ther., 206, 384–388 (1973).
    CAS Google Scholar
  4. Godfraind, T., and Lesne, M., Br. J. Pharmac., 46, 488–497 (1972).
    Article CAS Google Scholar
  5. Cohen, I., Daut, J., and Noble, D., J. Physiol., Lond., 260, 75–103 (1976).
    Article CAS Google Scholar
  6. Ghysel-Burton, J., and Godfraind, T., Br. J. Pharmac., 55, 249P (1975).
    CAS Google Scholar
  7. Baker, P. F., and Willis, J. S., Nature, 226, 521–523 (1970).
    Article ADS CAS Google Scholar
  8. Brading, A. F., and Widdicombe, J. H., J. Physiol., Lond., 238, 235–249 (1974).
    Article CAS Google Scholar
  9. Godfraind, T., Biochem. Pharmac., 24, 823–827 (1975).
    Article CAS Google Scholar
  10. Lamb, J. F., and McCall, D., J. Physiol., Lond., 225, 599–617 (1972).
    Article CAS Google Scholar
  11. Colqhoun, D., Drug Receptors (edit. by Rang, H. P.,) 149–182 (Macmillan, London and Basingstoke, 1973).
    Book Google Scholar
  12. Cavieres, J. D., and Ellory, J. C., Nature, 255, 338–340 (1975).
    Article ADS CAS Google Scholar
  13. Glynn, I. M., and Karlish, S. J. D., J. Physiol., Lond., 256, 465–496 (1976).
    Article CAS Google Scholar
  14. Godfraind, T., J. Physiol., Lond., 260, 21–35 (1976).
    Article CAS Google Scholar

Download references

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Authors and Affiliations

  1. Laboratoire de Pharmacodynamie Générale et de Pharmacologie, Université Catholique de Louvain, Avenue E. Mounier 73, UCL 7350, 1200, Bruxelles, Belgium
    T. GODFRAIND & J. GHYSEL-BURTON

Authors

  1. T. GODFRAIND
  2. J. GHYSEL-BURTON

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GODFRAIND, T., GHYSEL-BURTON, J. Binding sites related to ouabain-induced stimulation or inhibition of the sodium pump.Nature 265, 165–166 (1977). https://doi.org/10.1038/265165a0

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