Tumour-specific phenylalanine tRNA contains two supernumerary methylated bases (original) (raw)

Nature volume 271, pages 126–129 (1978) Cite this article

Abstract

Every malignant tumour examined contains aberrant tRNA methyltransferases and a few tRN As which are absent from the normal tissue of origin. To determine whether tumour-specific tRN As have different modifications from those in normal tissue, we purified the most frequently occurring tumour-specific isoaccepting tRNA from two malignant tissues. The isoaccepting phenylalanine tRNA from Novikoff hepatoma and Ehrlich ascites cells both contain two supernumerary methylated bases. One of these 1-methylguanine, is absent from the phenylalanine tRNA of normal rat, mouse, rabbit and calf liver. An increase in the levels of 5-methylcytidine and dihydrouridine was also detected.

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Authors and Affiliations

  1. Department of Microbiology, University of Colorado Medical Center, Denver, Colorado, 80262
    Yoshiyuki Kuchino & Ernest Borek
  2. Biology Division, National Cancer Research Institute, Tokyo, Japan
    Yoshiyuki Kuchino

Authors

  1. Yoshiyuki Kuchino
  2. Ernest Borek

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Kuchino, Y., Borek, E. Tumour-specific phenylalanine tRNA contains two supernumerary methylated bases.Nature 271, 126–129 (1978). https://doi.org/10.1038/271126a0

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