Protein transfer across microsomal membranes reassembled from separated membrane components (original) (raw)

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• Published: 15 June 1978

Nature volume 273, pages 569–571 (1978)Cite this article

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Abstract

THE synthesis of secretory proteins is initiated on ribosomes in the cell cytoplasm and the first part of the polypeptide chain to appear is a short sequence termed the signal sequence1. This signal is thought to direct the ribosomal complex to the endoplasmic reticulum (ER) membrane where the synthesis of the rest of the secretory protein is tightly coupled to its transfer across the membrane into the cisternal space. In all but one case, it is clear that the polypeptide chain is processed during transfer to remove the signal sequence2,3. Little is known about the membrane proteins involved in conveying the growing polypeptide chain across the membrane. A classical approach to learning more about these proteins would be to dissect the microsomal membrane into inactive components that can be reassembled subsequently into a functional entity. This would allow the purification of components involved in protein transfer. Here we report the first such reassembly.

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References

1. Blobel, G. & Dobberstein, B. J. Cell Biol. 67, 835–851, 852–867 (1975).
   Article CAS PubMed Google Scholar
2. Campbell, P. N. & Blobel, G. FEBS Lett. 72, 215–226 (1976).
   Article CAS PubMed Google Scholar
3. Palmiter, R. D., Thibodeau, S. N., Gagon, J. & Walsh, K. A. 11th FEBS Mtg, Copenhagen, 1977, (Pergamon, London, 1977).
   Google Scholar
4. Kreibich, G., Pereyra, B. & Sabatini, D. D. Abstr. Tenth Int. Congr. Biochem., Hamburg, 297 (1976).
5. Dobberstein, B. & Blobel, G. Biochem. biophys. Res. Commun. 74, 1675–1782 (1977).
   Article CAS PubMed Google Scholar
6. Sabatini, D. D. & Blobel, G. J. Cell Biol. 45, 146–157 (1970).
   Article CAS PubMed PubMed Central Google Scholar
7. Adelman, M. R., Blobel, G. & Sabatini, D. D. J. Cell Biol. 56, 191–205 (1973).
   Article CAS PubMed PubMed Central Google Scholar
8. Mans, R. J. & Novelli, G. D. Archs. Biochem. Biophys. 94, 48–53 (1961).
   Article CAS Google Scholar
9. Bartlett, G. R. J. biol. Chem. 234, 466–471 (1959).
   CAS PubMed Google Scholar
10. Lowry, O. H., Rosebrough, N. J., Farr, R. J. & Randall, R. J. J. biol. Chem. 193, 265–275 (1951).
    CAS PubMed Google Scholar

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Authors and Affiliations

1. European Molecular Biology Laboratory Postfach 10.2209 D6900 Heidelberg FRG,
   GRAHAM WARREN & BERNHARD DOBBERSTEIN

Authors

1. GRAHAM WARREN
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2. BERNHARD DOBBERSTEIN
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WARREN, G., DOBBERSTEIN, B. Protein transfer across microsomal membranes reassembled from separated membrane components.Nature 273, 569–571 (1978). https://doi.org/10.1038/273569a0

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• Received: 12 December 1977
• Accepted: 13 March 1978
• Issue Date: 15 June 1978
• DOI: https://doi.org/10.1038/273569a0

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