A different approach to RNA sequencing (original) (raw)

Nature volume 274, pages 87–89 (1978)Cite this article

Abstract

DURING the past few years techniques to simplify the systematic sequence determination of nucleic acids have become available; these involve the use of polyacrylamide gels from which the sequence may be directly and rapidly read off. The most recent of these, applicable to RNA fragments, rely on the base-specific nature of cleavage by _T_1 and _U_2 RNases1,2 and pancreatic RNase and RNase I from _Physarum polycephalum_2 by which a spectrum of 5′ terminally-labelled fragments may be produced and comigrated with a ‘ladder’ achieved by incubating the fragment with alkali or hot formamide. We describe here a method for the sequence determination of RNA fragments bearing a blocked 5′ terminus, in which the above enzymatic hydrolysis steps and their accompanying problems may be avoided.

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Author notes

  1. JOHN STANLEY
    Present address: Department of Molecular Biology, Agricultural University, De Dreijen 11, Wageningen, The Netherlands

Authors and Affiliations

  1. Institut de Biologie Moléculaire et Cellulaire du CNRS Université Louis Pasteur, 15 rue Descartes, 67084, Strasbourg, France
    JOHN STANLEY
  2. Novosibizsk State University, Novosibizsk, 90, USSR
    STANISLAV VASSILENKO

Authors

  1. JOHN STANLEY
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  2. STANISLAV VASSILENKO
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STANLEY, J., VASSILENKO, S. A different approach to RNA sequencing.Nature 274, 87–89 (1978). https://doi.org/10.1038/274087a0

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