Aphidicolin prevents mitotic cell division by interfering with the activity of DNA polymerase-α (original) (raw)

Nature volume 275, pages 458–460 (1978)Cite this article

Abstract

THE study of the control of DNA synthesis would be helped if a variety of chemicals which influenced DNA synthesis in various ways was available. We have searched for chemicals which prevent mitotic division of sea urchin embryos, which requires DNA synthesis, but do not prevent meiotic divisions of starfish oocytes, which are independent of DNA synthesis1. Aphidicolin2 (I) is one such compound. Furthermore, recent experiments have shown that aphidicolin selectively inhibits the activity of DNA polymerase-α obtained from regenerating rat liver without interfering with the activity of DNA polymerase-β and mitochondrial DNA polymerase3. The function of the DNA polymerases in the complex process of DNA replication is not known. Correlative studies of DNA synthesis and the level of DNA polymerase activity suggest that DNA polymerase-α may be important in DNA replication4. However, in certain cases, DNA polymerase-β and -γ also increase at the time of DNA synthesis, and attempts to specify which of the DNA polymerases is ‘replicative’ or to assign roles to the various polymerases have not yet been successful4. We report here evidence that prevention of cell division by aphidicolin in sea urchin embryos is due to selective inhibition of DNA polymerase-α activity. This study implies a functional role for this polymerase activity during replication of DNA.

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References

  1. Zampetti-Bosseler, F., Huez, G. & Brachet, J. Expl Cell Res. 78, 383–393 (1973).
    Article CAS Google Scholar
  2. Dalziel, W., Hesp, B., Stevenson, K. M. & Jarvis, J. A. J. J. chem. Soc. Perkin Trans. I, 2841–2851 (1973).
  3. Ohashi, M., Taguchi, T. & Ikegami, S. Biochem. biophys. Res. Commun. 82, 1084–1090 (1978).
    Article CAS Google Scholar
  4. Weissbach, A. A. Rev. Biochem. 46, 25–47 (1977).
    Article CAS Google Scholar
  5. Brachet, J. & Malpoix, P. Adv. Morphogenesis 9, 263–316 (1971).
    Article CAS Google Scholar
  6. Ikegami, S., Kamiya, Y. & Shirai, H. Expl Cell Res. 103, 233–239 (1976).
    Article CAS Google Scholar
  7. Mathews, C. K. Expl Cell Res. 92, 47–56 (1975).
    Article CAS Google Scholar
  8. Hinegardner, R. T., Rao, B. & Feldman, D. E. Expl Cell Res. 36, 51–61 (1964).
    Article Google Scholar
  9. Mano, Y. Devl Biol. 22, 433–460 (1970).
    Article CAS Google Scholar
  10. Loeb, L. A. J. biol. Chem. 244, 1672–1681 (1969).
    CAS PubMed Google Scholar
  11. Suzuki-Hori, C., Nagano, H. & Mano, Y. J. Biochem., Tokyo 82, 1613–1621 (1977).
    Article CAS Google Scholar

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Authors and Affiliations

  1. Department of Agricultural Chemistry, University of Tokyo, Bunkyo-ku, Tokyo, 113, Japan
    SUSUMU IKEGAMI
  2. Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology, Itabashi-ku, Tokyo, 173
    TAKAHIKO TAGUCHI & MOCHIHIKO OHASHI
  3. Department of Physiological Chemistry and Nutrition, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Tokyo, 113, Japan
    MIEKO OGURO, HIROSHI NAGANO & YOSHITAKE MANO

Authors

  1. SUSUMU IKEGAMI
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  2. TAKAHIKO TAGUCHI
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  3. MOCHIHIKO OHASHI
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  4. MIEKO OGURO
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  5. HIROSHI NAGANO
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  6. YOSHITAKE MANO
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IKEGAMI, S., TAGUCHI, T., OHASHI, M. et al. Aphidicolin prevents mitotic cell division by interfering with the activity of DNA polymerase-α.Nature 275, 458–460 (1978). https://doi.org/10.1038/275458a0

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