Nucleotide sequences of the attachment sites of bacteriophage Mu DNA (original) (raw)
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- Published: 01 July 1980
Nature volume 280, pages 247–250 (1979)Cite this article
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Abstract
THE temperate bacteriophage Mu has the remarkable ability to insert its DNA in apparently random sites of the Escherichia coli chromosome (1–3). All Mu prophages have the same gene order4, and the finding that the prophage and the phage maps are identical suggests that specific sites (attachment sites) at the ends of the Mu genome are used for the integration of Mu5. Phage particles do not contain the excised, free form of the Mu DNA; instead, the Mu genome is flanked by what seems to be heterogeneous bacterial DNA6,7. The variable DNA at both ends of Mu is thought to be generated when Mu DNA is transposed to many new chromosomal sites during replication and is subsequently packaged into phage particles together with adjacent host DNA. The nucleotide sequence analysis of the ends of Mu DNA reported here substantiates this hypothesis and identifies the attachment sites of Mu. Structural features of the attachment sites are presented.
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References
- Taylor, A. J. Proc. natn. Acad. Sci. U.S.A. 50, 1043–1051 (1963).
Article ADS CAS Google Scholar - Bukhari, A. I. & Zipser, D. Nature new Biol. 236, 240–243 (1972).
Article Google Scholar - Daniell, E., Roberts, R. J. & Abelson, J. J. molec. Biol. 69, 1–8 (1972).
Article CAS Google Scholar - Abelson, J. et al. Virology 54, 90–92 (1973).
Article CAS Google Scholar - Hsu, M.-T. & Davidson, N. Virology 58, 229–239 (1974).
Article CAS Google Scholar - Daniell, E., Kohne, D. E. & Abelson, J. J. Virol. 15, 739–743 (1975).
CAS PubMed PubMed Central Google Scholar - Bukhari, A. I., Froshauer, S. & Botchan, M. Nature 264, 580–583 (1976).
Article ADS CAS Google Scholar - Zipser, D., Moses, P., Kahmann, R. & Kamp, D. Gene 2, 263–271 (1977).
Article CAS Google Scholar - Maxam, A. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).
Article ADS CAS Google Scholar - O'Day, K. J., Schultz, D. W. & Howe, M. M. Microbiology (ed. Schlessinger, D.), 48–51 (American Society for Microbiology, 1978).
Google Scholar - Ohtsubo, H. & Ohtsubo, E. Proc. natn. Acad. Sci. U.S.A. 75, 615–619.
- Grindley, N. D. F. Cell 13, 419–426 (1978).
Article CAS Google Scholar - Rosenberg, M., Court, D., Shimatake, H., Brady, C. & Wulff, D. L. Nature 272, 414 (1978).
Article ADS CAS Google Scholar - Calos, M. P., Johnsrud, L. & Miller, J. H. Cell 13, 411–418 (1978).
Article CAS Google Scholar - Ohtsubo, H., Ohmori, H. & Ohtsubo, E. Cold Spring Harb. Symp. quant. Biol. 43 (1979).
- Kamp, D. et al. Cold Spring Harb. Symp. quant. Biol. 43 (1979).
- Allett, B. Cell 16, 123–129 (1979).
Article Google Scholar - Allet, B. Nature 274, 553–558 (1978).
Article ADS CAS Google Scholar - Kahmann, R., Kamp, D. & Zipser, D. DNA Insertion Elements, Plasmids and Episomes (eds Bukhari, A. I., Shapiro, J. A. & Adhya, S. L.) (Cold Spring Harbor, New York, 1977).
Google Scholar - Chow, L. T., Kahmann, R. & Kamp, D. J. molec. Biol. 113, 591–609 (1977).
Article CAS Google Scholar - Chow, L. T. & Broker, T. R. J. Bact. 133, 1427–1436 (1978).
CAS PubMed Google Scholar - Bolivar, R. et al. Gene 2, 95–113 (1977).
Article CAS Google Scholar - Murray, N. E. & Murray, K. Nature 251, 476–481 (1974).
Article ADS CAS Google Scholar
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- Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 11724
REGINE KAHMANN & DIETMAR KAMP
Authors
- REGINE KAHMANN
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KAHMANN, R., KAMP, D. Nucleotide sequences of the attachment sites of bacteriophage Mu DNA.Nature 280, 247–250 (1979). https://doi.org/10.1038/280247a0
- Received: 24 April 1979
- Accepted: 18 May 1979
- Published: 01 July 1980
- Issue Date: 19 July 1979
- DOI: https://doi.org/10.1038/280247a0
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