Platelet-derived growth factor prevents G0 growth arrest (original) (raw)
- Letter
- Published: 01 October 1979
Nature volume 281, pages 390–392 (1979)Cite this article
- 75 Accesses
- 49 Citations
- Metrics details
Abstract
Baserga1 has summarised evidence that there are two growth states in which cells have a diploid content of DNA; G1—the interval between mitosis and S phase in exponentially growing cultures—and G0, a quiescent state entered only when conditions for growth are suboptimal. G0 can be distinguished from G1 by temporal measurements; for BALB/c 3T3 cells, the lag time between G0 and S phase is never shorter than 12 h (ref. 2), whereas in exponentially growing cells the mean lag time between mitosis and S (G1 by definition) lasts 5–6 h (ref. 3). G0 can also be distinguished from G1 by some biochemical parameters including G0- and G1-specific intracellular proteins4,5. SV40- or polyoma virus-transformed cells cannot enter G0 to become quiescent6–9. Serum induces the growth of BALB/c 3T3 cells in tissue culture. It sustains the growth of exponentially replicating populations, and causes density-inhibited cells to leave G0 and replicate10–12. Serum contains several sets of hormonal growth factors which have recently been defined2,13,14. One of these hormones, the platelet-derived growth factor (PDGF), is released into serum during the clotting process; PDGF is absent in platelet-poor plasma, the liquid portion of unclotted blood15–19. It promotes the growth of G0-arrested cells2,16 by stimulating cells to become ‘competent’ to enter the S phase2; plasma allows these competent cells to progress through G0/G1, synthesise DNA2,20 and divide13. We now show that PDGF has a second function. It prevents replicating cells from entering G0.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Additional access options:
Similar content being viewed by others
References
- Augenlicht, L. & Baserga, R. Expl Cell Res. 89, 255–262 (1974).
Article CAS Google Scholar - Pledger, W. J., Stiles, C. D., Antoniades, H. N. & Scher, C. D. Proc. natn. Acad. Sci. U.S.A. 74, 4481–4485 (1977).
Article ADS CAS Google Scholar - Yen, A. & Pardee, A. B. Expl Cell Res. 116, 103–113 (1978).
Article CAS Google Scholar - Farmer, S. R., Ben-Ze'ev, A., Benecke, B. & Penman, S. Cell 15, 627–637 (1978).
Article CAS Google Scholar - Riddle, V. G. H., Dubrow, R. & Pardee, A. B. Proc. natn. Acad. Sci. U.S.A. 76, 1298–1302 (1979).
Article ADS CAS Google Scholar - Pardee, A. B. Proc. natn. Acad. Sci. U.S.A. 71, 1286–1290 (1974).
Article ADS CAS Google Scholar - Baserga, R., Costlow, M. & Rovera, G. Fedn Proc. 32, 2115–2118 (1973).
CAS Google Scholar - Burstin, S. J. & Basilico, C. Proc. natn. Acad. Sci. U.S.A. 72, 2540–2544 (1975).
Article ADS CAS Google Scholar - Martin, R. G. & Stein, S. Proc. natn. Acad. Sci. U.S.A. 73, 1655–1659 (1976).
Article ADS CAS Google Scholar - Todaro, G. J., Lazar, G. K. & Green, H. J. cell. comp. Physiol. 66, 325–334 (1965).
Article CAS Google Scholar - Holley, R. W. & Kiernan, J. A. Proc. natn. Acad. Sci. U.S.A. 60, 300–304 (1968).
Article ADS CAS Google Scholar - Brooks, R. F. Nature 260, 248–250 (1976).
Article ADS CAS Google Scholar - Vogel, A., Raines, E., Kariya, B., Rivest, M. J. & Ross, R. Proc. natn. Acad. Sci. U.S.A. 75, 2810–2814 (1978).
Article ADS CAS Google Scholar - Stiles, C. D. et al. Proc. natn. Acad. Sci. U.S.A. 76, 1279–1283 (1979).
Article ADS CAS Google Scholar - Ross, R., Glomset, J., Kariya, B. & Harker, L. Proc. natn. Acad. Sci. U.S.A. 71, 1207–1210 (1974).
Article ADS CAS Google Scholar - Rutherford, R. B. & Ross, R. J. Cell Biol. 69, 196–203 (1976).
Article CAS Google Scholar - Kohler, N. & Lipton, A. Expl Cell Res. 87, 297–301 (1974).
Article CAS Google Scholar - Heldin, C. H., Wasteson, A. & Westermark, B. Expl Cell Res. 109, 429–437 (1977).
Article CAS Google Scholar - Antoniades, H. N., Scher, C. D. & Stiles, C. D. Proc. natn. Acad. Sci. U.S.A. 76, 1809–1813 (1979).
Article ADS CAS Google Scholar - Pledger, W. J., Stiles, C. D., Antoniades, H. N. & Scher, C. D. Proc. natn. Acad. Sci. U.S.A. 75, 2839–2843 (1978).
Article ADS CAS Google Scholar - Stiles, C. D., Isberg, R., Pledger, W. J., Antoniades, H. N. & Scher, C. D. J. cell. Physiol. 99, 395–406 (1979).
Article CAS Google Scholar - Shimke, R. T., Kaufman, R. J., Alt, F. W. & Kellems, R. F. Science 202, 1051–1055 (1978).
Article ADS Google Scholar - Brooks, R. F. Cell 12, 311–317 (1977).
Article CAS Google Scholar - Smith, J. A. & Martin, L. Proc. natn. Acad. Sci. U.S.A. 70, 1263–1267 (1973).
Article ADS CAS Google Scholar
Author information
Authors and Affiliations
- Department of Hematology-Oncology, Sidney Farber Cancer Institute and Children's Hospital Medical Center, Department of Pediatrics,
Charles D. Scher & Marilyn E. Stone - Laboratory of Tumor Biology, Sidney Farber Cancer Institute, Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts, 02115
Charles D. Stiles
Authors
- Charles D. Scher
You can also search for this author inPubMed Google Scholar - Marilyn E. Stone
You can also search for this author inPubMed Google Scholar - Charles D. Stiles
You can also search for this author inPubMed Google Scholar
Rights and permissions
About this article
Cite this article
Scher, C., Stone, M. & Stiles, C. Platelet-derived growth factor prevents G0 growth arrest.Nature 281, 390–392 (1979). https://doi.org/10.1038/281390a0
- Received: 09 July 1979
- Accepted: 14 August 1979
- Published: 01 October 1979
- Issue Date: 04 October 1979
- DOI: https://doi.org/10.1038/281390a0