Evidence for fixed charge in the nexus (original) (raw)

Nature volume 285, pages 101–102 (1980)Cite this article

Abstract

The nexus or gap junction has been characterized as a low-resistance junction as well as a highly permeable junctional membrane to many molecules1–6. The transfer of electrical current from one cell interior to another, the aqueous solubility of dyes used to trace cell to cell communication and the fact that these molecules move across the nexus more rapidly than the plasma membrane4–10 have led to the hypothesis of an aqueous channel in the junction. Both Ca2+ (ref. 11) and H+ (ref. 12) are thought to alter nexal membrane conductance, and a voltage-sensitive gate has been demonstrated within the junction13. Recently, Flagg-Newton _et al._14 have concluded that mammalian junctions may contain fixed charge or be of smaller diameter than arthropod junctions. Here, we have investigated these alternatives by examining the permeability of nexuses of septa of the median giant axon of _Lumbricus terrestris_1,15 with various derivatives of fluorescein. Both carboxyfluorescein and aminofluorescein were found to have depressed permeabilities relative to their predicted permeabilities based on molecular size and weight (MW). Fluorescein diffusion was significantly suppressed in axons pre-injected with aminofluorescein but carboxyfluorescein had no such effect (Table 1). These data suggest the existence of fixed anionic charge within the nexal channel which may have affinity for amino groups.

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Authors and Affiliations

  1. Department of Anatomical Sciences, School of Basic Health Sciences, Health Sciences Center, State University of New York at Stony Brook, New York, 11794
    Peter R. Brink & Maynard M. Dewey

Authors

  1. Peter R. Brink
  2. Maynard M. Dewey

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Brink, P., Dewey, M. Evidence for fixed charge in the nexus.Nature 285, 101–102 (1980). https://doi.org/10.1038/285101a0

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