Avian sarcoma virus-transforming protein, pp60src shows protein kinase activity specific for tyrosine (original) (raw)

Nature volume 285, pages 167–169 (1980) Cite this article

Abstract

The protein responsible for malignant transformation by avian sarcoma viruses (ASVs) has been identified as a phos-phoprotein of molecular weight 60,000 designated pp60src (refs 1–4). It has been suggested that this protein has a functional role in cellular transformation involving the phosphorylation of cellular proteins, for it was discovered that specific immunoprecipitates from ASV-transformed cells that contain pp60src catalysed the transfer of phosphate from [γ-32P]ATP to the heavy chain of rabbit immunoglobulin5,6. Additional studies involving the cell-free synthesis of the ASV src protein further demonstrated that the presence of the src polypeptide correlated with that presence of a phosphotransf erase activity7. Our studies, involving the biochemical purification of this protein, have demonstrated that the ASV-transforming gene product, pp60src, is itself a protein kinase. We have purified the pp60src protein approximately 5,000-fold using either conventional ion-exchange chromatography or immunoaffinity chromatography8,9. The resultant partially purified preparations contain a cyclic AMP-independent protein kinase activity8. We report here that the soluble phosphotransferase activity of partially purified pp60src results in the phosphorylation of exclusively tyrosine residues in a variety of proteins that serve as substrates.

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Authors and Affiliations

  1. Department of Pathology, University of Colorado, School of Medicine, Denver, Colorado, 80262
    Marc S. Collett, A. F. Purchio & R. L. Erikson

Authors

  1. Marc S. Collett
  2. A. F. Purchio
  3. R. L. Erikson

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Collett, M., Purchio, A. & Erikson, R. Avian sarcoma virus-transforming protein, pp60src shows protein kinase activity specific for tyrosine.Nature 285, 167–169 (1980). https://doi.org/10.1038/285167a0

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