A herpes simplex virus type 1 function continuously required for early and late virus RNA synthesis (original) (raw)
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- Published: 29 May 1980
Nature volume 285, pages 329–330 (1980)Cite this article
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Abstract
Controlled transcription of animal virus DNAs provides potentially useful models for elucidating the mechanisms which regulate eukaryotic gene expression. The progressive transcription of the herpes simplex virus type 1 (HSV-1) genome has been described previously1–3. Infection of permissive cells with HSV-1 in the presence of the protein synthesis inhibitor cycloheximide resulted in transcription of a restricted set of virus RNAs, referred to as the immediate early RNAs, which map within certain regions of the virus genome only3–5. Removal of cycloheximide led to the transcription of additional virus DNA sequences, which were expressed during the normal replicative cycle both at early and late times post-infection (before and after the onset of virus DNA replication, respectively) and which map throughout the virus genome3. Previously, we have described a temperature-sensitive mutant of HSV-1 Glasgow strain 17, ts K, which accumulated only the immediate early RNAs at the non-permissive temperature (NPT)5. Transfer of ts K-infected cells from NPT to the permissive temperature (PT), even in the absence of de novo protein synthesis, resulted in transcription of the DNA sequences expressed early and late post-infection5. This indicated the persistence at NPT of a non-functional immediate early polypeptide which on transfer to PT regained its function, required for progression from the immediate early to early stage of transcription. Here we demonstrate, by analysing the RNAs made in ts K-infected cells after transfer from PT to the NPT, that this polypeptide's function is required continuously for synthesis of HSV-1 early and late RNAs, thus identifying a control function essential for the expression of early and late HSV genetic information in eukaryotic cells.
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References
- Frenkel, N. & Roizman, B. Proc. natn. Acad. Sci. U.S.A. 69, 2654–2658 (1972).
Article ADS CAS Google Scholar - Swanstrom, R. I. & Wagner, E. K. Virology 60, 522–533 (1974).
Article CAS Google Scholar - Clements, J. B., Watson, R. J. & Wilkie, N. M. Cell 12, 275–285 (1977).
Article CAS Google Scholar - Jones, P. C., Howard, G. S. & Roizman, B. J. Virol. 21, 268–276 (1977).
CAS PubMed PubMed Central Google Scholar - Watson, R. J. & Clements, J. B. Virology 91, 364–379 (1978).
Article CAS Google Scholar - Watson, R. J., Preston, C. M. & Clements, J. B. J. Virol. 31, 42–52 (1979).
CAS PubMed PubMed Central Google Scholar - Clements, J. B., McLauchlan, J. & McGeoch, D. J. Nucleic Acids Res. 7, 77–91 (1979).
Article CAS Google Scholar - Watson, R. J. thesis, Univ. Glasgow (1979).
- Lowe, P. A. Virology 86, 577–580 (1978).
Article CAS Google Scholar - Costanzo, F., Campadelli-Fiume, G., Foà-Tomasi, L. & Cassai, E. J. Virol. 21, 996–1001 (1977).
CAS PubMed PubMed Central Google Scholar - Stow, N. D., Subak-Sharpe, J. H. & Wilkie, N. M. Virology 90, 1–11 (1978).
Article CAS Google Scholar - Preston, V. G. et al. J. Virol. 28, 499–517 (1979).
Google Scholar - Preston, C. M. J. Virol. 32, 357–369 (1979).
CAS PubMed PubMed Central Google Scholar - Southern, E. M. J. molec. Biol. 98, 503–533 (1975).
Article CAS Google Scholar - Crombie, I. K. thesis, Univ. Glasgow (1975).
- Hayward, G. S., Frenkel, N. & Roizman, B. Proc. natn. Acad. Sci. U.S.A. 72, 4243–4247 (1975).
Article ADS CAS Google Scholar - Delius, H. & Clements, J. B. J. gen. Virol. 33, 125–133 (1976).
Article CAS Google Scholar
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Author notes
- Roger J. Watson
Present address: Laboratory of Molecular Virology, National Cancer Institute, Bethesda, Maryland, 20205
Authors and Affiliations
- Institute of Virology, University of Glasgow, Church Street, Glasgow, G11 5JR, UK
Roger J. Watson & J. Barklie Clements
Authors
- Roger J. Watson
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Watson, R., Clements, J. A herpes simplex virus type 1 function continuously required for early and late virus RNA synthesis.Nature 285, 329–330 (1980). https://doi.org/10.1038/285329a0
- Received: 14 December 1979
- Accepted: 05 March 1980
- Issue Date: 29 May 1980
- DOI: https://doi.org/10.1038/285329a0