Identification of a tetrodotoxin-sensitive Na+ channel in a variety of fibroblast lines (original) (raw)
- Letter
- Published: 10 July 1980
Nature volume 286, pages 162–164 (1980)Cite this article
- 62 Accesses
- 63 Citations
- Metrics details
Abstract
The action potential Na+ ionophore of excitable cells can be activated either by alkaloid compounds such as veratridine, or by small polypeptide toxins extracted from scorpion venom or sea anemone1,2. One of the main features of this Na+ channel is that it is blocked by tetrodotoxin (TTX). However, we report here that during analysis of Na+ influx in resting fibroblasts, we found that a variety of fibroblast lines also possess a TTX receptor. Veratridine and sea anemone toxin act synergistically to stimulate Na+ influx 7 to 10-fold in hamster and rat fibroblasts. As in excitable cells, this toxin-stimulated Na+ influx is blocked by TTX. Addition of serum to hamster fibroblasts arrested in G0 stimulates Na+ influx three-fold. Observations that TTX does not prevent serum-activated Na+ influx, initiation of DNA synthesis and cell proliferation suggest that the fast Na+ channel which we have identified in fibroblasts is not involved in growth control.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Additional access options:
Similar content being viewed by others
References
- Narahashi, T. Physiol. Rev. 54, 813–889 (1974).
Article CAS Google Scholar - Lazdunski, M. et al. in Function and Molecular Aspects of Biomembrane Transport (eds Quagliariello, E., Palmieri, F., Papa, S. & Klinkenberg, M.) 25–42 (Eisevier, Amsterdam, 1979).
Google Scholar - Catterall, W. A. & Nirenberg, M. Proc. natn. Acad. Sci. U.S.A. 70, 3759–3763 (1973).
Article ADS CAS Google Scholar - Fosset, M., De Barry, J., Lenoir, M. C. & Lazdunski, M. J. biol. Chem. 252, 6112–6117 (1977).
CAS PubMed Google Scholar - Jacques, Y., Fosset, M. & Lazdunski, M. J. biol. Chem. 253, 7383–7392 (1978).
CAS PubMed Google Scholar - Romey, G., Jacques, Y., Schweitz, H., Fosset, M. & Lazdunski, M. Biochim. biophys. Acta 556, 344–353 (1979).
Article CAS Google Scholar - Seif, R. & Cuzin, F. J. Virol. 24, 721–728 (1977).
CAS PubMed PubMed Central Google Scholar - Koch, K. & Leffert, H. Nature 279, 104–105 (1979).
Article ADS CAS Google Scholar - Pérez, R., Franchi, A. & Pouysségur, J. 2nd int. Cell Biol. Meet., Berlin (in the press).
Author information
Authors and Affiliations
- Centre de Biochimie, Faculté des Sciences, Parc Valrose, 06034, Nice, France
J. Pouysségur, Y. Jacques & M. Lazdunski
Authors
- J. Pouysségur
You can also search for this author inPubMed Google Scholar - Y. Jacques
You can also search for this author inPubMed Google Scholar - M. Lazdunski
You can also search for this author inPubMed Google Scholar
Rights and permissions
About this article
Cite this article
Pouysségur, J., Jacques, Y. & Lazdunski, M. Identification of a tetrodotoxin-sensitive Na+ channel in a variety of fibroblast lines.Nature 286, 162–164 (1980). https://doi.org/10.1038/286162a0
- Received: 18 February 1980
- Accepted: 07 May 1980
- Issue Date: 10 July 1980
- DOI: https://doi.org/10.1038/286162a0