Correlation of DNA exonic regions with protein structural units in haemoglobin (original) (raw)

Nature volume 291, pages 90–92 (1981)Cite this article

Abstract

The discovery of intervening sequences (introns) in DNA led Gilbert and Tonegawa1,2 to suggest that a new protein could have been produced by bringing together certain segments of pre-existing ones. However, Blake3 argued that if DNA was so organized that coding sequences (exons) correspond to structural as well as functional units of proteins, then combinations would be much more likely to yield a stable globular conformation through being ‘sums of parts’. In immunoglobulin heavy chain, four separate exons encode four different units4, all with distinct functions and three of which have clear domain structures. However, in haemoglobin, which has no obvious domain structure, no clear conformational characteristics have so far been recognized for the segments encoded by exons. From a close inspection of their conformations by drawing various stereodiagrams and the Cα–Cα distance map, I now propose a conformational characterization of the segments as structural units.

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References

  1. Gilbert, W. Nature 271, 501 (1978).
    Article ADS CAS Google Scholar
  2. Tonegawa, S., Maxam, A. M., Tizard, R., Bernard, O. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 75, 1485–1489 (1978).
    Article ADS CAS Google Scholar
  3. Blake, C. C. F. Nature 273, 267 (1978).
    Article ADS Google Scholar
  4. Sakano, H. et al. Nature 277, 627–633 (1979).
    Article ADS CAS Google Scholar
  5. Leder, A. et al. Proc. natn. Acad. Sci. U.S.A. 75, 6187–6191 (1978).
    Article ADS CAS Google Scholar
  6. Nishioka, Y. & Leder, P. Cell 18, 875–882 (1979).
    Article CAS Google Scholar
  7. Konkel, D. A., Tilghman, S. M. & Leder, P. Cell 15, 1125–1132 (1978).
    Article CAS Google Scholar
  8. Konkel, D. A., Maizel, J. V. Jr & Leder, P. Cell 18, 865–873 (1979).
    Article CAS Google Scholar
  9. Craik, C. S., Buchman, S. R. & Beychok, S. Proc. natn. Acad. Sci. U.S.A. 77, 1384–1388 (1980).
    Article ADS CAS Google Scholar
  10. Eaton, W. A. Nature 284, 183–185 (1980).
    Article ADS CAS Google Scholar
  11. Rao, S. T. & Rossmann, M. G. J. molec. Biol. 76, 241–246 (1973).
    Article CAS Google Scholar
  12. Wetlaufer, D. B. Proc. natn. Acad. Sci. U.S.A. 70, 697–701 (1973).
    Article ADS CAS Google Scholar
  13. Rossmann, M. G. & Liljas, A. J. molec. Biol. 85, 177–181 (1974).
    Article Google Scholar
  14. Gō, M. & Miyazawa, S. Int. J. Peptide Protein Res. 15, 211–224 (1980).
    Article Google Scholar
  15. Nishikawa, K., Ooi, T., Isogai, Y. & Saito, N. J. phys. Soc. Japan 32, 1331–1337 (1974).
    Article ADS Google Scholar
  16. Kuntz, I. D. J. Am. chem. Soc. 97, 4362–4366 (1975).
    Article CAS Google Scholar
  17. Jung, A., Sippel, A. E., Grez, M. & Schütz, G. Proc. natn. Acad. Sci. U.S.A. 77, 5759–5763 (1980).
    Article ADS CAS Google Scholar
  18. Anfinsen, C. B. & Scheraga, H. A. Adv. Protein Chem. 29, 205–300 (1975).
    Article CAS Google Scholar
  19. Doolittle, W. F. Nature 272, 581–582 (1978).
    Article ADS Google Scholar
  20. Perutz, M. F., Muirhead, H., Cox, J. M. & Goaman, L. C. G. Nature 219, 131–139 (1968).
    Article ADS CAS Google Scholar
  21. Dayhoff, M. O., Hunt, L. T., McLaughlin, P. J. & Barker, W. C. Atlas of Protein Sequence and Structure Vol. 5 (ed. Dayhoff, M. O.) D370–D371 (National Biomedical Foundation, Maryland, 1972).
    Google Scholar

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  1. Department of Biology, Faculty of Science, Kyushu University, 33, Fukuoka, 812, Japan
    Mitiko Gō

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Gō, M. Correlation of DNA exonic regions with protein structural units in haemoglobin.Nature 291, 90–92 (1981). https://doi.org/10.1038/291090a0

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