Monoclonal antibodies against human fibroblast interferon (original) (raw)

Nature volume 291, pages 500–501 (1981)Cite this article

Abstract

The advantages of using monoclonal antibodies account for current interest in antibodies specific for interferem1–3. The successful isolation of a hybridoma line secreting mouse monoclonal antibodies to human leukocyte interf eron (IFN-α) has already been reported4. We have now established a stable hybrid myeloma line (FO) which secretes antibody against human fibroblast interferon (IFN-β). We have demonstrated that, even after removal of much of the sugar moiety of IFN-β, the monoclonal anti-IFN-β antibodies neutralize the antiviral activity of human IFN-β but not that of human IFN-α. We suggest the possible use of these monoclonal antibodies for the identification and purification of IFN-β expressed by DNA cloned in bacteria.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 51 print issues and online access

$199.00 per year

only $3.90 per issue

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

Similar content being viewed by others

References

  1. Köhler, G. & Milstein, C. Nature 256, 495–497 (1975).
    Article ADS Google Scholar
  2. Köhler, G. & Milstein, C. Eur. J. Immun. 6, 511–519 (1976).
    Article Google Scholar
  3. Köhler, G., Hengartner, H. & Shulman, M. J. Eur. J. Immun. 8, 82–88 (1978).
    Article Google Scholar
  4. Secher, D. S. & Burke, D. C. Nature 285, 446–450 (1980).
    Article ADS CAS Google Scholar
  5. Menge, U. & Kula, M. R. German patent application No. P 2943016.8.
  6. Havell, E. A. & Vilcek, J. Antimicrob. Agents Chemother. 2, 476–484 (1972).
    Article CAS Google Scholar
  7. de Fazekas, S. E., & Scheidegger, S. J. immun, meth. 35, 1–25 (1980).
    Article Google Scholar
  8. Towbin, H., Staehelin, T. & Gordon, J. Proc. natn, Acad, Sci. U.S.A. 76, 4350–4354 (1979).
    Article ADS CAS Google Scholar
  9. Laemmli, U. K. Nature 227, 680–685 (1970).
    Article ADS CAS Google Scholar
  10. Weissenbach, J. et al. Proc. natn. Acad. Sci. U.S.A. 77, 7152–7156 (1980).
    Article ADS CAS Google Scholar
  11. Sagar, A. D., Pickering, L. A., Sussman-Berger, P., Stewart, W. E. Pickering, L. A., Sussman-Berger, P., Stewart, W. E. II & Sehgal, P. B. Nucleic Acids Res. 9, 149–160 (1981).
    Article CAS Google Scholar
  12. Bowen, B., Steinberg, J., Laemmli, U. K. & Weintraub, H. Nucleic Acids Res. 8, 1–19 (1980).
    Article CAS Google Scholar
  13. Hunter, W. M. & Greenwood, I. C. Nature 194, 495–496 (1962).
    Article ADS CAS Google Scholar

Download references

Author information

Authors and Affiliations

  1. Gesellachaft für Biotechnologische Forshung mbH, Mascheroder Weg 1, D–3300, Braunschweig, FRG
    H. K. Hochkeppel, U. Menge & J. Collins

Authors

  1. H. K. Hochkeppel
    You can also search for this author inPubMed Google Scholar
  2. U. Menge
    You can also search for this author inPubMed Google Scholar
  3. J. Collins
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

Hochkeppel, H., Menge, U. & Collins, J. Monoclonal antibodies against human fibroblast interferon.Nature 291, 500–501 (1981). https://doi.org/10.1038/291500a0

Download citation

This article is cited by