Antibody directed at a surface structure inhibits cytolytic but not suppressor function of human T lymphocytes (original) (raw)

Nature volume 294, pages 168–170 (1981)Cite this article

Abstract

Experiments using conventional antisera1,2 and monoclonal antibodies3–5 have shown that there are two functionally distinct human T-cell subsets which express unique cell-surface glycoproteins6–9. The inducer population expresses a 62,000-molecular weight (_M_r) antigen termed T4 (ref. 6) whereas the cytotoxic/suppressor population lacks T4 but expresses T5 antigen. This antigen is a 76,000-_M_r molecule in nonreducing conditions and a 33,000-_M_r glycoprotein occasionally appearing as a doublet in reducing conditions. As the functions of subset-specific T-cell surface glycoproteins have not been elucidated, we have now examined the effect on T-cell function of three monoclonal antibodies reactive with the cytotoxic/suppressor subset; anti-T8A antibody markedly inhibited cell-mediated lympholysis (CML) and anti-T8 partially affected CML, whereas anti-T5 had no effect. In contrast, there was no effect on suppressor cell function. Immunoprecipitation studies and competitive binding experiments indicated that anti-T8 and anti-T8A, like anti-T5, defined a 33,000-_M_r antigen. Taken together, our results suggest that of a possible family of 33,000-_M_r glycoproteins on the human cytotoxic/suppressor T-lymphocyte subset, that which is reactive with anti-T8A has some effect on the cytolytic mechanism.

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Authors and Affiliations

  1. Division of Tumor Immunology and Department of Medicine, Sidney Farber Cancer Institute, Harvard Medical School, 44 Binney Street, Boston, Massachusetts, 02115, USA
    Ellis L. Reinherz, Rebecca E. Hussey, Kathleen Fitzgerald, Peter Snow, Cox Terhorst & Stuart F. Schlossman

Authors

  1. Ellis L. Reinherz
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  2. Rebecca E. Hussey
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  3. Kathleen Fitzgerald
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  4. Peter Snow
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  5. Cox Terhorst
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  6. Stuart F. Schlossman
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Reinherz, E., Hussey, R., Fitzgerald, K. et al. Antibody directed at a surface structure inhibits cytolytic but not suppressor function of human T lymphocytes.Nature 294, 168–170 (1981). https://doi.org/10.1038/294168a0

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