Evidence for expression of the paternal genome in the two-cell mouse embryo (original) (raw)
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- Published: 03 December 1981
Nature volume 294, pages 450–451 (1981)Cite this article
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Abstract
Although there is strong evidence that the paternal genome is activated at the four- to eight-cell stage of mouse embryogenesis1–12, studies to date have been restricted because of their dependence on the manifestations of gene products, such as enzyme activity and cell-surface expression, rather than on direct assay of their synthesis. _β_2-microglobulin (_β_2M), a 12,000-molecular weight (_M_r) peptide associated with several of the gene products encoded by the H_–2–_Tla complex in the mouse13–17, is synthesized by early cleavage stage embryos (J.A.S., T.M. and C.J.E., unpublished). The existence of an electrophoretic variant of _β_2M in inbred strains has recently been described18–20, and tryptic peptide mapping19 and amino acid sequencing data21 indicate that the primary sequences of these variants differ by a single amino acid, thus establishing the genetic basis for this variation. Mice heterozygous at the _β_2m locus synthesize both forms, _β_2Ma and _β_2Mb (refs 18, 20). Using direct immunoprecipitation and two-dimensional gel electrophoresis, we have used this variation at the _β_2m locus to distinguish between maternal and paternal _β_2M and have now established that the synthesis of paternally derived _β_2M is first detectable at the two-cell stage. This is the earliest stage at which expression of the mammalian embryonic genome has been established22.
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References
- Epstein, C. J. Science 175, 1467–1468 (1972).
Article ADS CAS PubMed Google Scholar - Brinster, R. L. Biochem. Genet. 9, 187–191 (1973).
Article CAS PubMed Google Scholar - Krco, C. J. & Goldberg, E. H. Transplant Proc. 9, 1367–1370 (1977).
CAS PubMed Google Scholar - Muggleton-Harris, A. L. & Johnson, M. H. J. Embryol. exp. Morph. 35, 59–72 (1976).
CAS PubMed Google Scholar - Wudl, L. & Sherman, M. I. Cell 9, 523–531 (1976).
Article CAS PubMed Google Scholar - Adler, D. A., West, J. D. & Chapman, V. M. Nature 267, 838–839 (1977).
Article ADS CAS PubMed Google Scholar - Epstein, C. J., Smith, S., Travis, B. & Tucker, G. Nature 274, 500–503 (1978).
Article ADS CAS PubMed Google Scholar - Kratzer, P. G. & Gartler, S. M. Nature 274, 503–504 (1978).
Article ADS CAS PubMed Google Scholar - Monk, M. in Development in Mammals Vol. 1 (ed. Johnson, M. H.) 189–223 (North-Holland, Amsterdam, 1978).
Google Scholar - Monk, M. & Harper, M. J. Embryol. exp. Morph. 46, 53–64 (1978).
CAS PubMed Google Scholar - Epstein, C. J., Smith, S. & Travis, B. Tissue Antigens 15, 63–67 (1980).
Article CAS PubMed Google Scholar - Esworthy, S. & Chapman, V. M. Devl Genet. 2, 1–12 (1981).
Article CAS Google Scholar - Rask, L., Lindblom, B. & Peterson, P. A. Nature 249, 833–834 (1974).
Article ADS CAS PubMed Google Scholar - Silver, J. & Hood, L. Nature 249, 764–765 (1974).
Article ADS CAS PubMed Google Scholar - Armeding, D., Kubo, R. T., Grey, H. M. & Katz, D. H. Proc. natn. Acad. Sci. U.S.A. 72, 4577–4581 (1975).
Article ADS Google Scholar - Capra, J. D., Vitetta, E. & Klein, J. J. exp. Med. 142, 664–672 (1975).
Article CAS PubMed Google Scholar - Vitetta, E., Uhr, J. W. & Boyse, E. A. J. Immun. 114, 252–254 (1975).
CAS PubMed Google Scholar - Michaelson, J., Rothenberg, E. & Boyse, E. A. Immunogenetics 11, 93–95 (1980).
Article CAS PubMed Google Scholar - Robinson, P. J., Graf, L. & Sege, K. Proc. natn. Acad. Sci. U.S.A. 78, 1167–1170 (1981).
Article ADS CAS Google Scholar - Goding, J. W. & Walker, I.D. Proc. natn. Acad. Sci. U.S.A. 77, 7395–7399 (1980).
Article ADS CAS Google Scholar - Gates III, F. T., Coligan, J. E. & Kindt, T. J. Proc. natn. Acad. Sci. U.S.A. 78, 554–558 (1981).
Article ADS CAS Google Scholar - Magnuson, T. R. & Epstein, C. J. Biol. Rev. 56, 369–408 (1981).
Article CAS PubMed Google Scholar - Sawicki, J. A. & Epstein, C. J. Immunogenetics 12, 141–151 (1981).
Article CAS PubMed Google Scholar - O'Farrell, P. H. J. biol. Chem. 250, 4007–4021 (1975).
CAS PubMed Google Scholar - O'Farrell, P. Z., Goodman, H. M. & O'Farrell, P. H. Cell 12, 1133–1142 (1977).
Article CAS PubMed Google Scholar - Epstein, C. J., Wegienka, A. & Smith, C. W. Biochem. Genet. 3, 271–281 (1969).
Article CAS PubMed Google Scholar - Golbus, M. S. & Epstein, C. J. Differentiation 2, 143–149 (1974).
Article CAS PubMed Google Scholar
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Authors and Affiliations
- Departments of Pediatrics and of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, 94143, USA
Janet A. Sawicki, Terry Magnuson & Charles J. Epstein
Authors
- Janet A. Sawicki
You can also search for this author inPubMed Google Scholar - Terry Magnuson
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Sawicki, J., Magnuson, T. & Epstein, C. Evidence for expression of the paternal genome in the two-cell mouse embryo.Nature 294, 450–451 (1981). https://doi.org/10.1038/294450a0
- Received: 29 June 1981
- Accepted: 16 October 1981
- Issue Date: 03 December 1981
- DOI: https://doi.org/10.1038/294450a0