Product of a transferred H–2Ld gene acts as restriction element for LCMV-specific killer T cells (original) (raw)

Nature volume 297, pages 415–417 (1982)Cite this article

Abstract

The major histocompatibility complex (MHC) of the mouse, H–2 on chromosome 17, contains several families of genes encoding cell-surface molecules which have a role in mediating immune responses1,2. The class I genes encode a family of homologous membrane proteins including the transplantation antigens K, D and L. These antigens reflect extensive genetic polymorphism which is apparent in the many different class I gene constellations or haplotypes found in mice (for example, BALB/c mice exhibit the H–2 d haplotype and their class I molecules are denoted Kd, DD and Ld). Transplantation antigens serve as targets for T-cell killing in allogeneic immune responses such as in vivo graft rejection3 and destruction of allogeneic cells by cytotoxic T cells _in vitro_4. However, the physiological role of transplantation antigens may be to serve as restricting elements in virus-mediated T-cell killing of infected self cells. Virus infection of mice generates killer T cells whose receptors must interact with the foreign viral antigen and a class I molecule or restricting element for the cytotoxic effector function to be activated5,6. Thus the T-cell receptor recognizes the viral antigen in the context of a class I molecule. To study the interaction between the T-cell receptor and the class I restricting element, we have used the mouse L-cell transformant 8–5 which expresses Ld molecules7 and the K7-65 transformant expressing K molecules (R.S.G. et al., in preparation). Mouse L cells are fibroblasts derived from C3H mice of H–2 k haplotype and monoclonal antibodies can be used to distinguish H–2 d molecules from the endogenous H–2 k products. Recently, we have demonstrated that both Ld (ref. 8) and Kd molecules (unpublished data) expressed on transformed L cells can act as target antigens for alloreactive cytotoxic T lymphocytes. Here we show that the Ld molecule on transformed mouse L cells can serve as a restricting element in lymphocytic choriomeningiris virus (LCMV) infection, whereas its Kd counterpart cannot.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 51 print issues and online access

$199.00 per year

only $3.90 per issue

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

Similar content being viewed by others

References

  1. Klein, J. Science 203, 516–521 (1979).
    Article ADS CAS Google Scholar
  2. Snell, G. D. Science 213, 172–178 (1981).
    Article ADS CAS Google Scholar
  3. Snell, G. D., Dausset, J. & Nathenson, S., (eds) Histocompatibility (Academic, New York, 1976).
  4. Alter, B. J. et al. J. exp. Med. 137, 1303–1309 (1973).
    Article CAS Google Scholar
  5. Doherty, P. C. & Zinkernagel, R. M. J. exp. Med. 141, 502–507 (1975).
    Article CAS Google Scholar
  6. Zinkernagel, R. M. & Doherty, P. C. Adv. Immun. 27, 51–177 (1979).
    Article CAS Google Scholar
  7. Goodenow, R. S. et al. Science 215, 677–679 (1982).
    Article ADS CAS Google Scholar
  8. Woodward, J. G. et al. Proc. natn. Acad. Sci. U.S.A. (in the press).
  9. Stern, P., Martin, G. R. & Evans, M. J. Cell 6, 455–465 (1975).
    Article Google Scholar
  10. Biddison, W. E., Hansen, T. H., Levy, R. B. & Doherty, P. C. J. exp. Med. 148, 1678–1686 (1978).
    Article CAS Google Scholar
  11. Blanden, R. V., McKenzie, I. F. C., Kees, U., Melvold, R. W. & Kohn, H. I. J. exp. Med. 146, 869–880 (1977).
    Article CAS Google Scholar
  12. Levy, R. B., Shearer, G. M. & Hansen, T. H. J. Immun. 121, 2263–2269 (1978).
    CAS PubMed Google Scholar
  13. McKenzie, I. F. C., Morgan, G. M., Melvold, R. W. & Kohn, H. I. Immunogenetics 4, 333–347 (1977).
    Article Google Scholar
  14. Hansen, T. H. et al. J. Immun. 126, 1713–1716 (1981).
    CAS PubMed Google Scholar
  15. Hansen, T. H. & Sachs, D. H. J. Immun. 121, 1469–1472 (1978).
    CAS PubMed Google Scholar
  16. Huang, C.-M., Huang, S. & Klein, J. Immunogenetics 9, 173–182 (1979).
    Article Google Scholar
  17. Ozato, K., Hansen, T. H. & Sachs, D. H. J. Immun. 125, 2473–2477 (1980).
    CAS PubMed Google Scholar
  18. Woodward, J. G. et al. in B and T Cell Tumors: Biological and Clinical Aspects (eds Vitetta, E. & Fox, C. F.) (Academic, New York, in the press).
  19. Ozato, K., Meyer, R. & Sachs, D. H. Transplantation (in the press).

Download references

Author information

Authors and Affiliations

  1. Division of Biology, California Institute of Technology, Pasadena, California, 91125, USA
    Anders Örn, Robert S. Goodenow & Leroy Hood
  2. Department of Neurology, University of Southern California Medical School, Los Angeles, California, 90033, USA
    Peter R. Brayton
  3. Department of Microbiology, University of Southern California Medical School, Los Angeles, California, 90033, USA
    Jerold G. Woodward, Richard C. Harmon & Jeffrey A. Frelinger

Authors

  1. Anders Örn
    You can also search for this author inPubMed Google Scholar
  2. Robert S. Goodenow
    You can also search for this author inPubMed Google Scholar
  3. Leroy Hood
    You can also search for this author inPubMed Google Scholar
  4. Peter R. Brayton
    You can also search for this author inPubMed Google Scholar
  5. Jerold G. Woodward
    You can also search for this author inPubMed Google Scholar
  6. Richard C. Harmon
    You can also search for this author inPubMed Google Scholar
  7. Jeffrey A. Frelinger
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

Örn, A., Goodenow, R., Hood, L. et al. Product of a transferred H–2L d gene acts as restriction element for LCMV-specific killer T cells.Nature 297, 415–417 (1982). https://doi.org/10.1038/297415a0

Download citation