Molecular cloning of the gene for human anti-haemophilic factor IX (original) (raw)
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- Published: 09 September 1982
Nature volume 299, pages 178–180 (1982)Cite this article
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Abstract
A functional deficiency of factor IX, one of the coagulation factors involved in blood clotting1, leads to the bleeding disorder known as Christmas disease2, or haemophilia B. Both this disease and haemophilia A (factor VIII (C) deficiency) are X chromosome-linked and together occur at a frequency of ∼1 in 10,000 males. The molecular basis for the functional alteration of factor IX in Christmas disease is not clearly understood. As a first step towards the elucidation of the molecular events involved, we have attempted molecular cloning of the factor IX gene. We used a bovine factor IX cDNA clone, isolated using synthetic oligonucleotides as probes, to screen a cloned human gene library. Here we report the isolation and partial characterization of a λ recombinant phage containing the human factor IX gene.
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Authors and Affiliations
- Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK
K. H. Choo, K. G. Gould, D. J. G. Rees & G. G. Brownlee
Authors
- K. H. Choo
You can also search for this author inPubMed Google Scholar - K. G. Gould
You can also search for this author inPubMed Google Scholar - D. J. G. Rees
You can also search for this author inPubMed Google Scholar - G. G. Brownlee
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Choo, K., Gould, K., Rees, D. et al. Molecular cloning of the gene for human anti-haemophilic factor IX.Nature 299, 178–180 (1982). https://doi.org/10.1038/299178a0
- Received: 12 May 1982
- Accepted: 23 June 1982
- Issue Date: 09 September 1982
- DOI: https://doi.org/10.1038/299178a0