Differential expression of steroid sulphatase locus on active and inactive human X chromosome (original) (raw)

Nature volume 299, pages 838–840 (1982)Cite this article

Abstract

The X chromosome in mammalian somatic cells is subject to unique regulation—usually genes on a single X chromosome are expressed while those on other X chromosomes are inactivated1. The X-locus for steroid sulphatase (STS; EC 3.1.6.2), the microsomal enzyme that catalyses the hydrolysis of various 3_β_-hydroxysteroid sulphates, is exceptional because it seems to escape inactivation. Evidence for this comes from fibroblast clones in females heterozygous for mutations that result in a severe deficiency of this enzyme in affected males; all clones from these heterozygotes have STS activity, and enzyme-deficient clones that are expected if the locus were subject to inactivation2, have not been found3. Further evidence that the STS locus escapes inactivation is that the human inactive X chromosome contributes STS activity to mouse–human hybrid cells4. On the basis of these hybrid studies5,6 the STS locus has been mapped to the distal half of the short arm (p22–pter) of the human X chromosome. Although the STS locus on both X chromosomes in human female cells is expressed, quantitative measurements of STS activity in males and females do not accurately reflect the sex differences in number of X chromosomes7–13 (Table 1). The ratio of mean values for normal females to that of normal males is greater than 1:1 but less than the ratio of 2:1 expected if STS loci on all X chromosomes were equally expressed. The incomplete dosage effect suggests that the STS locus on the inactive X chromosome might not be fully expressed. To test this hypothesis, we examined two heterozygotes for X-linked STS deficiency who were also heterozygous for the common electrophoretic variants of glucose-6-phosphate dehydrogenase (G6PD A and B). Studies of fibroblast clones from these females provide evidence, presented here, for differential expression of STS loci on the active and inactive X chromosome.

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Authors and Affiliations

  1. Department of Pediatrics, The Johns Hopkins University School of Medicine, Baltimore, Maryland, 21205, USA
    Barbara R. Migeon, Joyce Axelman & Rebecca L. Dabora
  2. Division of Medical Genetics, Harbor–UCLA Medical Center, Torrance, California, 90509, USA
    Larry J. Shapiro & Thuluvancheri Mohandas
  3. Department of Medicine, Henry Ford Hospital, Detroit, Michigan, 48202, USA
    Robert A. Norum

Authors

  1. Barbara R. Migeon
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  2. Larry J. Shapiro
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  3. Robert A. Norum
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  4. Thuluvancheri Mohandas
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  5. Joyce Axelman
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  6. Rebecca L. Dabora
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Migeon, B., Shapiro, L., Norum, R. et al. Differential expression of steroid sulphatase locus on active and inactive human X chromosome.Nature 299, 838–840 (1982). https://doi.org/10.1038/299838a0

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