Inelastic neutron scattering analysis of hexokinase dynamics and its modification on binding of glucose (original) (raw)
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- Published: 04 November 1982
Nature volume 300, pages 84–86 (1982)Cite this article
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Abstract
The dynamical behaviour of proteins and its significance to protein function has become the subject of considerable experimental and theoretical investigation1,2. The existence of atomic motion about mean positions has been well established by the temperature dependence of X-ray diffraction3 and it has long been recognized that considerable internal mobility is required in proteins to account for hydrogen exchange measurements4. However, only limited experimental information, mainly obtained by optical methods5, is available on the frequencies associated with these fluctuations. Inelastic neutron scattering is a technique capable of yielding dynamical information about a system over a wide frequency range6,7. Recent advances in neutron spectrometers8 and in the theoretical understanding of the dynamics of biological macromolecules have now made it both feasible and desirable to exploit this technique in the field of biophysics. To demonstrate the potential of the method, we report here data on the frequency distribution in solution of the enzyme hexokinase and its modification brought about on binding of its substrate glucose. The results lend support to the view that ligation induces a stiffening of the enzyme structure.
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References
- Careri, G., Fasella, P. & Gratton, E. A. Rev. Biophys. Bioengng 8, 69–97 (1979).
Article CAS Google Scholar - Karplus, M. & McCammon, J. A. CRC Crit. Rev. Biochem. 9, 293–349 (1981).
Article CAS Google Scholar - Frauenfelder, H., Petsko, G. A. & Tsernoglou, D. Nature 280, 558–563 (1979).
Article ADS CAS Google Scholar - Rosa, J. J. & Richards, F. M. J. molec. Biol. 133, 399–416 (1979).
Article CAS Google Scholar - Peticolas, W. L. Meth. Enzym. 61, 421–458 (1978).
Google Scholar - Lovesey, S. W. & Springer, T. (eds) Dynamics of Solids and Liquids by Neutron Scattering (Springer, Berlin, 1977).
Google Scholar - Boutin, H. & Yip, S. Molecular Spectroscopy with Neutrons (MIT Press, 1968).
Google Scholar - Neutron Beam Facilities Available for Users (Institut Laue-Langevin, Grenoble, 1981).
- Bartunik, H. D., Jolles, P., Berthou, J. & Dianoux, A. J. Biopolymers 21, 43–50 (1982).
Article CAS Google Scholar - Anderson, C. M., Stenkamp, R. E. & Steitz, T. A. J. molec. Biol. 123, 15–33 (1978).
Article CAS Google Scholar - Bennett, W. S. & Steitz, T. A. Proc. natn. Acad. Sci. U.S.A. 75, 4848–4852 (1978).
Article ADS CAS Google Scholar - Bennett, W. S. & Steitz, T. A. J. molec. Biol. 140, 211–230 (1980).
Article CAS Google Scholar - McDonald, R. C., Steitz, T. A. & Engelman, D. M. Biochemistry 18, 338–342 (1979).
Article CAS Google Scholar - Sturtevant, J. M. Proc. natn. Acad. Sci. U.S.A. 74, 2236–2240 (1977).
Article ADS CAS Google Scholar - McCammon, J. A., Gelin, B. R., Karplus, M. & Wolynes, P. G. Nature 262, 325–326 (1976).
Article ADS CAS Google Scholar - Takahashi, K., Casey, J. L. & Sturtevant, J. M. Biochemistry 20, 4693–4697 (1981).
Article CAS Google Scholar - McCammon, J. A., Gelin, B. R. & Karplus, M. Nature 267, 585–590 (1977).
Article ADS CAS Google Scholar - Karplus, M. & McCammon, J. A. Nature 277, 578 (1979).
Article ADS CAS Google Scholar - Tasumi, M., Takeuchi, H., Ataka, S., Dwivedi, A. M. & Krimm, S. Biopolymers 21, 713 (1982).
Article Google Scholar
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Authors and Affiliations
- European Molecular Biology Laboratory, Grenoble Outstation, c/o ILL, 156X, 38042, Grenoble Cedex, France
Bernard Jacrot & Stephen Cusack - Institut Laue-Langevin, 156X, 38042, Grenoble Cedex, France
Albert J. Dianoux - Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, 06520, USA
Donald M. Engelman
Authors
- Bernard Jacrot
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Jacrot, B., Cusack, S., Dianoux, A. et al. Inelastic neutron scattering analysis of hexokinase dynamics and its modification on binding of glucose.Nature 300, 84–86 (1982). https://doi.org/10.1038/300084a0
- Received: 21 June 1982
- Accepted: 15 September 1982
- Issue Date: 04 November 1982
- DOI: https://doi.org/10.1038/300084a0
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