Elucidation by FAB–MS of the structure of a new cardioactive peptide from Aplysia (original) (raw)

• Letter
• Published: 16 December 1982
• Maria Panico1,
• Andrew Karplus1 nAff2,
• Philip E. Lloyd1 nAff3 &
• …
• Bernhard Riniker1 nAff4

Nature volume 300, pages 643–645 (1982) Cite this article

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Abstract

The nervous system and gut of Aplysia and other gastropod molluscs contain several classes of cardioactive peptides1. In addition to their excitatory action on the heart, these peptides stimulate contractile activity in the gut1, and modulate neurally induced contractions of somatic muscles2. This report describes the purification and sequence determination by a combination of microprotein chemistry and fast atom bombardment mass spectrometry FAB–MS3,4 of one of these peptides. The sequenced peptide is a member of the small class of cardioactive peptides and was termed SCPB. A peptide with identical properties to SCPB has been found in several identified central neurones which innervate the gut5. Here we report the structure of SCPB as a C-terminally blocked peptide H-Met-Asn-Tyr-Leu-Ala-Phe-Pro-Arg-Met-NH2 Native and synthetic SCPB have identical actions on molluscan muscle preparations with thresholds at around 10−10 M.

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Author notes

1. Andrew Karplus
   Present address: Department of Biochemistry, University of Washington, Seattle, Washington, 98159, USA
2. Philip E. Lloyd
   Present address: Centre for Neurology and Behaviour, College of Physicians and Surgeons, Columbia University, New York, New York, 10032, USA
3. Bernhard Riniker
   Present address: CIBA-Geigy A. G., Basel, Switzerland

Authors and Affiliations

1. Department of Biochemistry, Imperial College, London, SW7 2AZ, UK
   Howard R. Morris, Maria Panico, Andrew Karplus, Philip E. Lloyd & Bernhard Riniker

Authors

1. Howard R. Morris
2. Maria Panico
3. Andrew Karplus
4. Philip E. Lloyd
5. Bernhard Riniker

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Morris, H., Panico, M., Karplus, A. et al. Elucidation by FAB–MS of the structure of a new cardioactive peptide from Aplysia.Nature 300, 643–645 (1982). https://doi.org/10.1038/300643a0

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• Received: 25 August 1982
• Accepted: 18 October 1982
• Issue date: 16 December 1982
• DOI: https://doi.org/10.1038/300643a0