Aberrant rearrangement of the κ light-chain locus involving the heavy-chain locus and chromosome 15 in a mouse plasmacytoma (original) (raw)

Nature volume 301, pages 425–427 (1983)Cite this article

Abstract

The creation of a functional antibody gene requires the precise recombination of gene segments initially separated on the chromosome. Frequently errors occur in the process, resulting in the formation of a non-functional gene. The non-functional genes can be generated by incomplete rearrangements1, frame-shifts2, or the use of pseudo V or J joining segments3. It is likely that these aberrant rearrangements arise by the same mechanism as is used in generating functional genes, a process which we have suggested may involve unequal sister chromatid exchange4. Aberrant rearrangements of immunoglobulin genes occur in normal lymphocytes and play a major part in allelic exclusion5. However, it has recently been suggested that aberrant rearrangements involving immunoglobulin and non-immunoglobulin genes may be involved in tumorigenesis. This suggestion has been stimulated by the frequent occurrence of translocations involving chromosomes known to carry immunoglobulin genes in B-cell malignancies. The rearrangement of non-immunoglobulin DNA to the heavy-chain locus has recently been reported6,7. Some aberrant rearrangements of the κ locus appear to be due to rearrangements to sites that do not include the conventional sequence for V gene segment joining8. Here we describe an aberrant κ rearrangement that has led to the joining of DNA from chromosomes 15, 6 and 12, and so appears to be the result of chromosomal translocations or transpositions. As 15/6 or 15/12 translocations have frequently been found in mouse plasmacytomas (as have analogous translocations in human lymphocyte tumours) this aberrant κ rearrangement may be unique to the plasmacytoma from which it was isolated.

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Author notes

  1. Brian G. VanNess
    Present address: Department of Biochemistry, University of Iowa, Iowa City, Iowa, 52242, USA

Authors and Affiliations

  1. The Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania, 19111, USA
    Brian G. VanNess, Marjorie Shapiro, Dawn E. Kelley, Robert P. Perry & Martin Weigert
  2. Department of Biochemistry, New York University, New York, 10016, USA
    Peter D'Eustachio
  3. Department of Biology, Yale University, New Haven, Connecticut, 06511, USA
    Frank Ruddle

Authors

  1. Brian G. VanNess
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  2. Marjorie Shapiro
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  3. Dawn E. Kelley
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  4. Robert P. Perry
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  5. Martin Weigert
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  6. Peter D'Eustachio
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  7. Frank Ruddle
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VanNess, B., Shapiro, M., Kelley, D. et al. Aberrant rearrangement of the κ light-chain locus involving the heavy-chain locus and chromosome 15 in a mouse plasmacytoma.Nature 301, 425–427 (1983). https://doi.org/10.1038/301425a0

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