Multiple mRNA species with distinct 3′ termini are transcribed from the β2-microglobulin gene (original) (raw)

Nature volume 302, pages 449–452 (1983)Cite this article

Abstract

β 2-Microglobulin is the small, relatively invariant subunit of a family of cell-surface glycoproteins encoded within the major histocompatibility complex (MHC). Proteins associated with β2-microglobulin in the mouse include the classical transplantation antigens (H–2K, D and L), the thymus leukaemia antigen (TL) and certain haematopoietic cell differentiation antigens (Qa-1 and Qa-2). The genes encoding these proteins are members of a large, multigene family1–3. In contrast, β2-microglobulin is encoded by a single copy gene4 on mouse chromosome 2 (refs 5, 6). We have shown that this gene consists of four coding blocks separated by three intervening sequences4. We now demonstrate that the single β2-microglobulin gene is transcribed into at least two different size classes of mRNA that differ in the lengths of their 3′ untranslated regions. We further show that three polyadenylation signals and a poly (A) tail are encoded at the 3′ end of the gene.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 51 print issues and online access

$199.00 per year

only $3.90 per issue

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

Similar content being viewed by others

References

  1. Steinmetz, M. et al. Cell 24, 125–134 (1981).
    Article CAS Google Scholar
  2. Cami, B., Bregegere, F., Abastado, J. P. & Kourilsky, P. Nature 291, 673–675 (1981).
    Article ADS CAS Google Scholar
  3. Margulies, D. H., Evans, G. A., Flaherty, L. & Seidman, J. G. Nature 295, 168–170 (1982).
    Article ADS CAS Google Scholar
  4. Parnes, J. R. & Seidman, J. G. Cell 29, 661–669 (1982).
    Article CAS Google Scholar
  5. Goding, J. W. J. Immun. 126, 1644–1666 (1981).
    CAS PubMed Google Scholar
  6. Michaelson, J. Immunogenetics 13, 167–171 (1981).
    Article CAS Google Scholar
  7. Croce, C. M. et al. Proc. natn. Acad. Sci. U.S.A. 78, 5754–5758 (1981).
    Article ADS CAS Google Scholar
  8. Morello, D. et al. Nature 296, 260–262 (1982).
    Article ADS CAS Google Scholar
  9. Battey, J. et al. Proc. natn. Acad. Sci. U.S.A. 79, 5956–5960 (1982).
    Article ADS CAS Google Scholar
  10. Hollis, G. F. et al. Nature 296, 321–325 (1982).
    Article ADS CAS Google Scholar
  11. Wilde, C. D. et al. nature 297, 83–84 (1982).
    Article ADS CAS Google Scholar
  12. Orr, H. T. et al. Nature 282, 266–270 (1979).
    Article ADS CAS Google Scholar
  13. Larhammar, D. et al. Proc. natn. Acad. Sci. U.S.A. 79, 3687–3691 (1982).
    Article ADS CAS Google Scholar
  14. Setzer, D. R., McGrogan, M., Nunberg, J. H. & Schimke, R. T. Cell 22, 361–370 (1980).
    Article CAS Google Scholar
  15. Tosi, M., Young, R. A., Hagenbüchle, O. & Schibler, U. Nucleic Acids Res. 9, 2313–2323 (1981).
    Article CAS Google Scholar
  16. Early, P. et al. Cell 22, 361–370 (1980).
    Article Google Scholar
  17. McKean, D. J. et al. J. exp. Med. 154, 1419–1431 (1981).
    Article CAS Google Scholar
  18. Parnes, J. R. et al. Proc. natn. Acad. Sci. U.S.A. 78, 2253–2257 (1981).
    Article ADS CAS Google Scholar
  19. Bailey, J. M. & Davidson, N. Analyt. Biochem. 70, 75–85 (1976).
    Article CAS Google Scholar
  20. Thomas, P. S. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1980).
    Article ADS CAS Google Scholar
  21. Wallace, R. A., Jared, D. W., Dumont, J. N. & Sega, M. W. J. exp. Zool. 184, 321–333 (1973).
    Article CAS Google Scholar
  22. Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).
    Article CAS Google Scholar
  23. Messing, J., Crea, R. & Seeburg, P. H. Nucleic Acids Res. 9, 309–321 (1981).
    Article CAS Google Scholar

Download references

Author information

Author notes

  1. Jane R. Parnes
    Present address: Department of Medicine, Stanford University Medical Center, Stanford, California, 94305, USA
  2. Randy R. Robinson
    Present address: Ingene, 1701 Colorado Avenue, Santa Monica, California, 90404, USA

Authors and Affiliations

  1. Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, 20205, USA
    Jane R. Parnes & Randy R. Robinson
  2. Department of Genetics, Harvard Medical School, Boston, Massachusetts, 02115, USA
    J. G. Seidman

Authors

  1. Jane R. Parnes
    You can also search for this author inPubMed Google Scholar
  2. Randy R. Robinson
    You can also search for this author inPubMed Google Scholar
  3. J. G. Seidman
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

Parnes, J., Robinson, R. & Seidman, J. Multiple mRNA species with distinct 3′ termini are transcribed from the β2-microglobulin gene.Nature 302, 449–452 (1983). https://doi.org/10.1038/302449a0

Download citation