Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphate (original) (raw)
- Letter
- Published: 03 November 1983
Nature volume 306, pages 67–69 (1983)Cite this article
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Abstract
Activation of receptors for a wide variety of hormones and neurotransmitters leads to an increase in the intracellular level of calcium. Much of this calcium is released from intracellular stores but the link between surface receptors and this internal calcium reservoir is unknown. Hydrolysis of the phosphoinositides, which is another characteristic feature of these receptors1–3, has been implicated in calcium mobilization1. The primary lipid substrates for the receptor mechanism seem to be two polyphosphoinositides, phosphatidylinositol 4-phosphate (PtdIns4P) and phosphatidylinositol 4,5-bisphosphate (PtdIns4,5P2), which are rapidly hydrolysed following receptor activation in various cells and tissues4–10. The action of phospholipase C on these polyphosphoinositides results in the rapid formation of the water-soluble products inositol 1,4-bisphosphate (Ins1,4P2) and inositol 1,4,5-trisphosphate (Ins1,4,5P3)9,11,12. In the insect salivary gland, where changes in Ins1,4P2 and Ins1,4,5P3 have been studied at early time periods, increases in these inositol phosphates are sufficiently rapid to suggest that they might mobilize internal calcium12. We report here that micromolar concentrations of Ins1,4,5P3 release Ca2+ from a nonmitochondrial intracellular Ca2+ store in pancreatic acinar cells. Our results strongly suggest that this is the same Ca2+ store that is released by acetylcholine.
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References
- Michell, R. H. Biochim. biophys. Acta 415, 81–147 (1975).
Article CAS Google Scholar - Berridge, M. J. Molec. cell. Endocr. 24, 115–140 (1981).
Article CAS Google Scholar - Hokin, M. R. & Hokin, L. E. J. biol. Chem. 203, 967–977 (1953).
CAS Google Scholar - Abdel-Latif, A. A., Akhtar, R. A. & Hawthorne, J. N. Biochem. J. 162, 61–73 (1977).
Article CAS Google Scholar - Kirk, C. J., Creba, J. A., Downes, P. & Michell, R. H. Biochem. Soc. Trans. 9, 377–379 (1981).
Article CAS Google Scholar - Thomas, A. P., Marks, J. S., Coll, K. E. & Williamson, J. R. J. biol. Chem. 258, 5716–5725 (1983).
CAS PubMed Google Scholar - Weiss, S. J., McKinney, J. S. & Putney, J. W. Biochem. J. 206, 555–560 (1982).
Article CAS Google Scholar - Billah, M. M. & Lapetina, E. G. J. biol. Chem. 257, 12705–12708 (1982).
CAS PubMed Google Scholar - Agranoff, B. W., Murthy, P. & Seguin, E. B. J. biol. Chem. 258, 2076–2078 (1983).
CAS Google Scholar - Putney, J. W., Burgess, G. M., Halenda, S. P., McKinney, J. S. & Rubin, R. P. Biochem. J. 212, 483–488 (1983).
Article CAS Google Scholar - Berridge, M. J., Dawson, R. M. C., Downes, C. P., Heslop, J. P. & Irvine, R. F. Biochem. J. 212, 473–482 (1983).
Article CAS Google Scholar - Berridge, M. J. Biochem. J. 212, 849–858 (1983).
Article CAS Google Scholar - Streb, H. & Schulz, I. Am. J. Physiol. (in the press).
- Affolter, H. & Sigel, E. Analyt. Biochem. 97, 315–319 (1979).
Article CAS Google Scholar - Tomlinson, R. V. & Ballou, C. E. J. biol. Chem. 236, 1902–1906 (1961).
CAS PubMed Google Scholar - Wakasugi, H., Kimura, T., Haase, W., Kribben, A., Kaufmann, R. & Schulz, I. J. Membrane Biol. 65, 205–220 (1982).
Article CAS Google Scholar - Lucas M., Galvan, A., Solano, P. & Goberna, R. Biochim. biophys. Acta 731, 129–136 (1982).
Article Google Scholar - Downes, C. P., Mussat, M. C. & Michell, R. H. Biochem. J. 203, 169–177 (1982).
Article CAS Google Scholar - Irvine, R. F., Hemington, N. & Dawson, R. M. C. Biochem. J. 176, 475–484 (1978).
Article CAS Google Scholar - Irvine, R. F., Letcher, A. J. & Dawson, R. M. C. Biochem. J. 178, 497–500 (1979).
Article CAS Google Scholar - Irvine, R. F., Letcher, A. J. & Dawson, R. M. C. Biochem. J. 192, 279–283 (1980).
Article CAS Google Scholar
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Authors and Affiliations
- Max-Planck-Institut für Biophysik, Kennedyallee 70, 6000, Frankfurt, (Main) 70, FRG
H. Streb & I. Schulz - Department of Biochemistry, ARC Institute of Animal Physiology, Babraham, Cambridge, CB2 4AT, UK
R. F. Irvine - ARC Unit of Insect Neurophysiology and Pharmacology, Department of Zoology, University of Cambridge, Downing Street, Cambridge, CB2 3EJ, UK
M. J. Berridge
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- H. Streb
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Streb, H., Irvine, R., Berridge, M. et al. Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphate.Nature 306, 67–69 (1983). https://doi.org/10.1038/306067a0
- Received: 28 June 1983
- Accepted: 25 August 1983
- Issue Date: 03 November 1983
- DOI: https://doi.org/10.1038/306067a0