Sequence of cDNA encoding human insulin-like growth factor I precursor (original) (raw)

Nature volume 306, pages 609–611 (1983)Cite this article

Abstract

Somatomedins (SM) or insulin-like growth factors (IGF) constitute a heterogeneous group of peptides1 with important growth-promoting effects _in vitro_2,3 as well as _in vivo_4,5. Amino acid sequences have been determined for only two of them, IGF-I and IGF-II, which are highly homologous6,7. IGF-I, which is identical with SM-C8, is composed of 70 amino acid residues and IGF-II contains 73 amino acids and may be identical with SM-A9. Other peptides with different charge properties but with similar SM-like or insulin-like behaviour in biological and receptor assays, have been described but have not yet been fully characterized1. The liver is known to be a major site of production of these peptides10, but many other tissues—especially in the fetus11—may synthesize them as well. We report here the nucleotide sequence of a human liver cDNA encoding the complete amino acid sequence of IGF-I. The IGF-I coding region is flanked by sequences encoding an amino-terminal peptide of at least 25 amino acid residues and a carboxyl-terminal peptide of 35 amino acids. This provides evidence that IGF-I is synthesized as a precursor protein and that formation of IGF-I from this precursor requires proteolytic processing at both ends.

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Authors and Affiliations

  1. Department of Pediatrics, State University of Utrecht, The Netherlands
    M. Jansen & J. L. Van den Brande
  2. Laboratory for Physiological Chemistry, State University of Utrecht, The Netherlands
    F. M. A. van Schaik & J. S. Sussenbach
  3. Department of Medicine, Children's Hospital Medical Center, Boston, Massachusetts, 02115, USA
    A. T. Ricker, B. Bullock, D. E. Woods & K. H. Gabbay
  4. Harvard Medical School, Boston, Massachusetts, 02115, USA
    A. T. Ricker, B. Bullock, D. E. Woods, K. H. Gabbay & A. L. Nussbaum

Authors

  1. M. Jansen
  2. F. M. A. van Schaik
  3. A. T. Ricker
  4. B. Bullock
  5. D. E. Woods
  6. K. H. Gabbay
  7. A. L. Nussbaum
  8. J. S. Sussenbach
  9. J. L. Van den Brande

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Jansen, M., van Schaik, F., Ricker, A. et al. Sequence of cDNA encoding human insulin-like growth factor I precursor.Nature 306, 609–611 (1983). https://doi.org/10.1038/306609a0

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