Direct observation of motion of single F-actin filaments in the presence of myosin (original) (raw)

Nature volume 307, pages 58–60 (1984)Cite this article

Abstract

Actin is found in almost all kinds of non-muscle cells where it is thought to have an important role in cell motility. A proper understanding of that role will only be possible when reliable in vitro systems are available for investigating the interaction of cellular actin and myosin. A start has been made on several systems1–4, most recently by Sheetz and Spudich who demonstrated unidirectional movement of HMM-coated beads along F-actin cables on arrays of chloroplasts exposed by dissection of a Nitella cell5. As an alternative approach, we report here the direct observation by fluorescence microscopy of the movements of single F-actin filaments interacting with soluble myosin fragments energized by Mg2+-ATP.

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References

  1. Oplatka, A. & Tirosh, R. Biochim. biophys. Acta 305, 683–688 (1973).
    Google Scholar
  2. Yano, M., Yamamoto & Shimizu, H. Nature 299, 557–559 (1982).
    Article ADS CAS Google Scholar
  3. Kuroda, K. & Kamiya, N. Proc. Japan. Acad. 51, 774–777 (1975).
    Article Google Scholar
  4. Higashi-Fujime, S. J. Cell Biol. 87, 569–578 (1980).
    Article CAS Google Scholar
  5. Sheetz, M. P. & Spudich, J. A. Nature 303, 31–35 (1983).
    Article ADS CAS Google Scholar
  6. Nagashima, H. & Asakura, S. J. molec. Biol. 136, 169–182 (1980).
    Article CAS Google Scholar
  7. Wulf, E., Deboben, A., Bautz, F. A., Faulstich, H. & Wieland, Th. Proc. natn. Acad. Sci. U.S.A. 76, 4498–4502 (1979).
    Article ADS CAS Google Scholar
  8. Pròchniewicz-Nakayama, E., Yanagida, T. & Oosawa, F. J. Cell Biol. (in the press).
  9. Fujime, S. J. Phys. Soc. Japan 29, 751–759 (1970).
    Article ADS CAS Google Scholar
  10. Yanagida, T. & Oosawa, F. J. molec. Biol. 126, 509–524 (1978).
    Article Google Scholar
  11. Yoshino, S., Umazume, Y., Natori, R., Fujime, S. & Chiba, S. Biophys. Chem. 8, 317–326 (1978).
    Article CAS Google Scholar
  12. Wieland, Th. & Faulstich, H. CRC crit. Rev. Biochem. 5, 185–260 (1978).
    Article CAS Google Scholar
  13. Eisenberg, E. & Moos, C. J. biol. Chem. 242, 2945–2951 (1967).
    CAS PubMed Google Scholar
  14. Knight, P. & Offer, G. Biochemistry 19, 4682–4687 (1980).
    Article CAS Google Scholar
  15. Yanagida, T. in Cross-bridge Mechanism in Muscle Contraction (eds Pollack, G. H. & Sugi, H.) (Plenum, New York, in the press).
  16. Landau, L. D. & Lifshitz, E. M. Statistical Physics, 478–482 (Pergamon, London, (1958).
    Google Scholar
  17. Harris, R. A. & Hearst, J. E. J. chem. Phys. 44, 2595–2609 (1966).
    Article ADS CAS Google Scholar

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Authors and Affiliations

  1. Department of Biophysical Engineering, Faculty of Engineering Science, Osaka University, Toyonaka, Osaka, Japan
    Toshio Yanagida, Michiyuki Nakase, Katsumi Nishiyama & Fumio Oosawa

Authors

  1. Toshio Yanagida
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  2. Michiyuki Nakase
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  3. Katsumi Nishiyama
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  4. Fumio Oosawa
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Yanagida, T., Nakase, M., Nishiyama, K. et al. Direct observation of motion of single F-actin filaments in the presence of myosin.Nature 307, 58–60 (1984). https://doi.org/10.1038/307058a0

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