Computer-based characterization of epidermal growth factor precursor (original) (raw)

Nature volume 307, pages 558–560 (1984)Cite this article

Abstract

The cDNA sequence of the precursor of mouse epidermal growth factor (EGFP) has recently been reported by two groups1,2, both of whom noted the presence of repeated similar segments, each about 40 residues long. One of these repeat units overlaps with the sequence of epidermal growth factor itself. The sequence of epidermal growth factor has been reported to be similar to that of pancreatic secretory trypsin inhibitor3 (PSTI) and a somewhat better match has been found with part of the sequence of bovine factor X4, one of the blood coagulating factors. We report here that there is an even stronger similarity between the sequences of some of the repeat units of epidermal growth factor precursor and certain segments in factor X. This sequence similarity is also apparent in comparisons with other blood coagulation factors. On the basis of these sequence comparisons we suggest a scheme for the evolution of the epidermal growth factor precursor. We have also identified certain structural features in the precursor sequence that bear on the way in which the mature epidermal growth factor is generated.

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References

  1. Gray, A., Dull, T. J. & Ullrich, A. Nature, 303, 722–725 (1983).
    Article ADS CAS Google Scholar
  2. Scott, J. et al. Science 221, 236–240 (1983).
    Article ADS CAS Google Scholar
  3. Hunt, L. T., Barker, W. & Dayhoff, M. O. Biochem. biophys. Res. Commun. 60, 1020–1028 (1974).
    Article CAS Google Scholar
  4. Dayhoff, M. O. Atlas of Protein Sequences and Structures Vol. 5 & Suppl. 1–3 (1978).
    Google Scholar
  5. Doolittle, R. F. Science 214, 149–159 (1981).
    Article ADS CAS Google Scholar
  6. Needleman, S. B. & Wunsch, C. D. J. molec. Biol. 48, 443–53 (1970).
    Article CAS Google Scholar
  7. Waterman, M. S., Smith, T. F. & Beyer, W. A. Adv. Math. 20, 367–387 (1976).
    Article Google Scholar
  8. Gotoh, O. J. molec. Biol. 162, 705–8 (1982).
    Article CAS Google Scholar
  9. Fitch, W. M. & Margoliash, E. Science 155, 279–284 (1967).
    Article ADS CAS Google Scholar
  10. Chou, P. Y. & Fasman, G. D. A. Rev. Biochem. 47, 251–276 (1978).
    Article CAS Google Scholar
  11. Garnier, J., Osguthorpe, D. J. & Robson, B. J. molec. Biol. 120, 97–120 (1978).
    Article CAS Google Scholar
  12. Savage, C. R., Hash, J. H. & Cohen, S. J. biol. Chem. 248, 7669–7672 (1973).
    CAS PubMed Google Scholar
  13. Katayama, K. et al. Proc. natn. Acad. Sci. U.S.A. 76, 4990–4994 (1979).
    Article ADS CAS Google Scholar
  14. Kyte, J. & Doolittle, R. F. J. molec. Biol. 157, 105–132 (1982).
    Article CAS Google Scholar
  15. Rose, J. K. et al. Proc. natn. Acad. Sci. U.S.A. 77, 3884–3888 (1980).
    Article ADS CAS Google Scholar
  16. Stern, D. M. et al. Proc. natn. Acad. Sci. U.S.A. 80, 4119–4123 (1983).
    Article ADS CAS Google Scholar
  17. Drakenberg, T. et al. Proc. natn. Acad. Sci. U.S.A. 80, 1802–1806 (1983).
    Article ADS CAS Google Scholar
  18. McMullen, B. A. et al. Biochemistry 22, 2875–2884 (1983).
    Article CAS Google Scholar
  19. Marquardt, H. et al. Proc. natn. Acad. Sci. U.S.A. 80, 4684–4688 (1983).
    Article ADS CAS Google Scholar

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Authors and Affiliations

  1. Department of Chemistry, University of California, San Diego, La Jolla, California, 92093, USA
    Russell F. Doolittle, Da Fei Feng & Mark S. Johnson

Authors

  1. Russell F. Doolittle
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  2. Da Fei Feng
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  3. Mark S. Johnson
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Doolittle, R., Fei Feng, D. & Johnson, M. Computer-based characterization of epidermal growth factor precursor.Nature 307, 558–560 (1984). https://doi.org/10.1038/307558a0

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