β2-Microglobulin from serum associates with MHC class I antigens on the surface of cultured cells (original) (raw)

Nature volume 308, pages 642–645 (1984)Cite this article

Abstract

_β_2-Microglobulin (_β_2-m) is a highly conserved polypeptide (12,000 molecular weight; 12K) noncovalently associated with the heavy chain (45–48K) of the major histocompatibility complex (MHC) class I antigens. Its synthesis is required for expression of the HLA-A/B and H–2K/D heavy chains at the cell surface1; _β_2-m is also associated with the human cell-surface antigens T6 and M241 isolated from thymocytes2–6. However, on the T leukaemic cell line MOLT-4 some of the T6 antigens contain a different 12K subunit, termed _β_t (refs 3, 7, 8). Purified human _β_2-m can exchange partially both with human _β_2-m associated with HLA-antigens9, and with mouse _β_2-m associated with murine alloantigens10, 11. As MOLT-4 cells were grown in the presence of fetal calf serum (FCS) and as serum is known to contain some free _β_2-m12, we examined whether _β_t was bovine β2-m which had replaced endogenous _β_2-m on the surface of the cell. Here we show both that _β_2-m from FCS or human serum (HuS) used in cell culture can exchange with _β_2-m on the cell surface, and that _β_t is in fact bovine _β_2-m.

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References

  1. Ploegh, H. L., Orr, H. T. & Strominger, J. L. Cell 24, 287–299 (1981).
    Article CAS Google Scholar
  2. Terhorst, C. et al. Cell 23, 771–780 (1981).
    CAS Google Scholar
  3. van Agthoven, A. & Terhorst, C. J. Immun. 128, 426–432 (1982).
    CAS PubMed Google Scholar
  4. Lerch, P. G., van de Rijn, M., Schrier, P. & Terhorst, C. Hum. Immun. 6, 13–30 (1983).
    Article CAS Google Scholar
  5. Knowles, R. W. & Bodmer, W. F. Eur. J. Immun. 12, 676 (1982).
    Article CAS Google Scholar
  6. van de Rijn, M., Lerch, P., Knowles, R. & Terhorst, C. J. Immun. 131, 851–855 (1983).
    CAS PubMed Google Scholar
  7. Ziegler, A. & Milstein, C. Nature 279, 243–244 (1979).
    Article ADS CAS Google Scholar
  8. Burrone, O. R., Calabi, F., Kefford, R. F. & Milstein, C. EMBO J. 2, 1591–1595 (1983).
    Article CAS Google Scholar
  9. Hyafil, F. & Strominger, J. L. Proc. natn. Acad. Sci. U.S.A. 76, 5834–5838 (1979).
    Article ADS CAS Google Scholar
  10. Schmidt, W., Festenstein, H., Ward, P. J. & Sanderson, A. R. Immunogenetics 13, 483–491 (1981).
    Article CAS Google Scholar
  11. Hester, R. B., Kubo, R. T. & Grey, H. M. Scand. J. Immun. 9, 125–134 (1979).
    Article CAS Google Scholar
  12. Berggard, I. & Bearn, A. G. J. biol. Chem. 243, 4095–4103 (1968).
    CAS PubMed Google Scholar
  13. Barnstable, C. J. et al. Cell 14, 9–20 (1978).
    Article CAS Google Scholar
  14. Cejka, J. & Kithier, K. Archs Biochem. Biophys. 153, 854–861 (1972).
    Article CAS Google Scholar
  15. Groves, M. L. & Greenberg, R. J. biol. Chem. 257, 2619–2626 (1982).
    CAS PubMed Google Scholar
  16. Bernabeu, C. et al. J. Immun. 131, 2032–2037 (1983).
    CAS PubMed Google Scholar
  17. Stallcup, K. C., Springer, T. A. & Mescher, M. F. J. Immun. 127, 923–927 (1981).
    CAS PubMed Google Scholar
  18. Laemmli, U. K. Nature 227, 680–685 (1970).
    Article ADS CAS Google Scholar
  19. Barbosa, J. A. et al. Proc. natn. Acad. Sci. U.S.A. 79, 6327–6331 (1982).
    Article ADS CAS Google Scholar
  20. Terhorst, C. P., Parham, P., Mann, D. L. & Strominger, J. L. Proc. natn. Acad. Sci. U.S.A. 73, 910–914 (1976).
    Article ADS CAS Google Scholar
  21. Shustik, C., Cohen, I. R., Schwartz, R. S., Latham-Griffin, E. & Waksal, S. D. Nature 263, 699–701 (1976).
    Article ADS CAS Google Scholar
  22. Forni, G. & Green, I. J. Immun. 116, 1561–1565 (1976).
    CAS PubMed Google Scholar
  23. Tsutsui, I. & Everett, N. B. Cell. Immun. 10, 359–370 (1974).
    Article CAS Google Scholar
  24. Peck, A. B., Anderson, L. C. & Wigzell, M. J. exp. Med. 145, 802–818 (1977).
    Article CAS Google Scholar
  25. Goodenow, R. S. et al. Nature 300, 231–235 (1982).
    Article ADS CAS Google Scholar

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Authors and Affiliations

  1. Laboratory of Molecular Immunology, Dana Farber Cancer Institute, 44 Binney Street, Boston, Massachusetts, 02115, USA
    Carmelo Bernabeu, Matthijs van de Rijn, Peter G. Lerch & Cox P. Terhorst

Authors

  1. Carmelo Bernabeu
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  2. Matthijs van de Rijn
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  3. Peter G. Lerch
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  4. Cox P. Terhorst
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Bernabeu, C., van de Rijn, M., Lerch, P. et al. _β_2-Microglobulin from serum associates with MHC class I antigens on the surface of cultured cells.Nature 308, 642–645 (1984). https://doi.org/10.1038/308642a0

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