β2-Microglobulin from serum associates with MHC class I antigens on the surface of cultured cells (original) (raw)
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- Published: 12 April 1984
Nature volume 308, pages 642–645 (1984)Cite this article
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Abstract
_β_2-Microglobulin (_β_2-m) is a highly conserved polypeptide (12,000 molecular weight; 12K) noncovalently associated with the heavy chain (45–48K) of the major histocompatibility complex (MHC) class I antigens. Its synthesis is required for expression of the HLA-A/B and H–2K/D heavy chains at the cell surface1; _β_2-m is also associated with the human cell-surface antigens T6 and M241 isolated from thymocytes2–6. However, on the T leukaemic cell line MOLT-4 some of the T6 antigens contain a different 12K subunit, termed _β_t (refs 3, 7, 8). Purified human _β_2-m can exchange partially both with human _β_2-m associated with HLA-antigens9, and with mouse _β_2-m associated with murine alloantigens10, 11. As MOLT-4 cells were grown in the presence of fetal calf serum (FCS) and as serum is known to contain some free _β_2-m12, we examined whether _β_t was bovine β2-m which had replaced endogenous _β_2-m on the surface of the cell. Here we show both that _β_2-m from FCS or human serum (HuS) used in cell culture can exchange with _β_2-m on the cell surface, and that _β_t is in fact bovine _β_2-m.
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Authors and Affiliations
- Laboratory of Molecular Immunology, Dana Farber Cancer Institute, 44 Binney Street, Boston, Massachusetts, 02115, USA
Carmelo Bernabeu, Matthijs van de Rijn, Peter G. Lerch & Cox P. Terhorst
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- Carmelo Bernabeu
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Bernabeu, C., van de Rijn, M., Lerch, P. et al. _β_2-Microglobulin from serum associates with MHC class I antigens on the surface of cultured cells.Nature 308, 642–645 (1984). https://doi.org/10.1038/308642a0
- Received: 10 October 1983
- Accepted: 19 December 1983
- Issue Date: 12 April 1984
- DOI: https://doi.org/10.1038/308642a0