H–2-linked low-molecular weight polypeptide antigens assemble into an unusual macromolecular complex (original) (raw)
- Letter
- Published: 28 June 1984
Nature volume 309, pages 797–799 (1984)Cite this article
Abstract
The major histocompatibility complex (MHC) is a cluster of tightly linked genes whose products are of central importance in the functioning of the immune system. Class I and II MHC antigens are integral membrane proteins which regulate cell-surface interactions between T cells and their targets1–3, while class III antigens are components of the complement system of serum proteins4. All available evidence indicates that the structure and function of the MHC and its gene products are highly conserved among species (for review, see ref. 5). We recently reported6 the existence in murine cells of a fourth class of MHC-linked polypeptides which are biochemically and genetically distinct from previously identified MHC gene products: BALB.B anti-BALB/c (anti-H–2d) antiserum immunoprecipitates a set of 16 cytoplasmic low-molecular weight polypeptides (LMP) from BALB/c spleen cells and from the WEHI-3 cell line. The production of these peptides is coordinately regulated (by immune interferon) with the production of the class I and II MHC antigens7,8, suggesting that they too are functionally relevant to the immune system. We demonstrate here that these 16 polypeptides are associated with one another in vivo as a very large (580,000-molecular weight, _M_r) noncovalent complex. The unusual nature of this complex has allowed the non-immunochemical identification of similar complexes from (serologically negative) H–2b murine cells and from a human cell line. Thus, LMP antigens display two properties in common with other MHC antigens: they are both polymorphic and genetically conserved across species.
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Authors and Affiliations
- Department of Medical Microbiology, Stanford University School of Medicine, Stanford, California, 94305, USA
John J. Monaco & Hugh O. McDevitt
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- John J. Monaco
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Monaco, J., McDevitt, H. H–2-linked low-molecular weight polypeptide antigens assemble into an unusual macromolecular complex.Nature 309, 797–799 (1984). https://doi.org/10.1038/309797a0
- Received: 23 December 1983
- Accepted: 25 April 1984
- Issue Date: 28 June 1984
- DOI: https://doi.org/10.1038/309797a0