Epidermal growth factor stimulates guanine nucleotide binding activity and phosphorylation of ras oncogene proteins (original) (raw)

Nature volume 310, pages 147–150 (1984)Cite this article

Abstract

Several human tumour cell lines contain genes that can transform NIH 3T3 cells into malignant cells. Certain genes have been classified as members of the ras oncogene family, namely, Ha-ras, Ki-ras or N-_ras_1–3. The proteins encoded by the ras family are generally small (Ha-ras, for example, encodes a protein of molecular weight 21,000 named p21), and are associated with the inner surface of the plasma membrane. The only known biochemical property common to all forms of the ras proteins is the ability to bind guanine nucleotides, a property which may be closely related to the transforming ability of ras proteins4,5. A GTP-dependent, apparent autophosphorylation (on threonine 59) activity has been identified only in the case of the _v_-Ha-ras protein6. Although the role of these biochemical activities in the transformation process remains unclear, we have initiated studies to determine the possible biochemical interactions of ras proteins with other membrane components. We report here the evidence that epidermal growth factor enhances the guanine nucleotide binding activity of activated _c_-Ha-ras or _v_-Ha-ras p21, and phosphorylation of _v_-Ha-ras p21, suggesting that some mitogenic growth factors may regulate those activities.

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  1. Cold Spring Harbor Laboratory, PO Box 100, Cold Spring Harbor, New York, 11724, USA
    Tohru Kamata & James R. Feramisco

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  1. Tohru Kamata
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Kamata, T., Feramisco, J. Epidermal growth factor stimulates guanine nucleotide binding activity and phosphorylation of ras oncogene proteins.Nature 310, 147–150 (1984). https://doi.org/10.1038/310147a0

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