High expression of cloned immunoglobulin k gene in transgenic mice is restricted to B lymphocytes (original) (raw)
- Letter
- Published: 19 July 1984
- Rebecca L. O'Brien1,
- Michael D. McMullen1,
- Katherine A. Gollahon1 &
- …
- Ralph L. Brinster2
Nature volume 310, pages 238–241 (1984)Cite this article
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Abstract
Immunoglobulin genes are normally expressed only in cells of the B lymphocyte lineage after a variable (V) and constant (C) gene rearrangement has occurred1. To study the control of immunoglobulin gene expression in a defined situation, we have produced transgenic mice by microinjecting a rearranged mouse immunoglobulin k gene (designated pB1-14) into fertilized mouse eggs2. We present here the analysis of six different _k_-transgenic mouse lines. All the transgenic mice express the microinjected k gene in a completely tissue-specific fashion. Transcripts from pB1-14 are found at a high level in the spleen, but are undetectable in nonlymphoid tissues of testis, liver, kidney, heart, muscle, brain and thyroid gland. In lymphoid cell subpopulations, the level of pB1-14 transcripts is correlated with the relative number of B cells; there is no correlation with the proportion of T lymphocytes. We concluded, therefore, that the microinjected k gene contains target sequences for B lymphocyte-specific gene activation signals that override the influence of the integration site.
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Authors and Affiliations
- Department of Microbiology and Immunology, University of Washington, Seattle, Washington, 98195, USA
Ursula Storb, Rebecca L. O'Brien, Michael D. McMullen & Katherine A. Gollahon - Laboratory of Reproductive Physiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, 19104, USA
Ralph L. Brinster
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- Ursula Storb
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Storb, U., O'Brien, R., McMullen, M. et al. High expression of cloned immunoglobulin k gene in transgenic mice is restricted to B lymphocytes.Nature 310, 238–241 (1984). https://doi.org/10.1038/310238a0
- Received: 03 April 1984
- Accepted: 25 April 1984
- Issue Date: 19 July 1984
- DOI: https://doi.org/10.1038/310238a0