Homology of Ti α-subunit of a T-cell antigen–MHC receptor with immunoglobulin (original) (raw)

Nature volume 312, pages 269–271 (1984) Cite this article

Abstract

Human T-cell receptors for antigen and major histocompatibility complex (MHC) determinants have now been defined on inducer1, suppressor2, and class 1 and class 2 MHC-specific cytotoxic T lymphocytes3 as T3-associated clonotypic molecules (Ti) of relative molecular mass 90,000 (90K) composed of one 49–54K _α_- and one 43K _β_-subunit which are disulphide-linked4. In the case of the Ti _β_-subunit, N-terminal amino acid sequencing5 and molecular cloning techniques6,7 led recently to identification of the Ti β-gene and showed that T-specific V, D, J and C segments fuse to form an active _β_-gene8,9. So far, however, there have been little structural data available on the Ti _α_-subunit. Here we have derived the amino acid sequence of a portion of the Ti _α_-subunit by CNBr fragmentation. Sequence analysis reveals ∼40% homology between the Ti _α_-subunit fragment and the third framework of the variable region of immunoglobulin light and heavy chains, supporting the notion that the Ti _α_-subunit is a member of the immunoglobulin–Ti _β_-gene family.

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Author notes

  1. Marina Fabbi
    Present address: Laboratorio di Immunobiologia, Istituto Nazionale per la Ricerca sul Cancro, Viale Benedetto XV, 10, 16132, Geneva, Italy

Authors and Affiliations

  1. Division of Tumor Immunology, Dana-Farber Cancer Institute and Departments of Medicine and Pathology, Harvard Medical School, Boston, Massachusetts, 02115, USA
    Marina Fabbi, Oreste Acuto & Ellis L. Reinherz
  2. Biogen Research Corporation, Cambridge, Massachusetts, 02142, USA
    John E. Smart

Authors

  1. Marina Fabbi
  2. Oreste Acuto
  3. John E. Smart
  4. Ellis L. Reinherz

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Fabbi, M., Acuto, O., Smart, J. et al. Homology of Ti _α_-subunit of a T-cell antigen–MHC receptor with immunoglobulin.Nature 312, 269–271 (1984). https://doi.org/10.1038/312269a0

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