Evidence for polarization of plasma membrane domains in pancreatic endocrine cells (original) (raw)

Nature volume 313, pages 694–696 (1985)Cite this article

Abstract

Polarization of plasma membrane domains is an essential feature of secretory epithelial cells from exocrine glands. The surface of exocrine cells (a typical example is the acinar cell of the pancreas) is separated into an apical domain, where secretion occurs by exocytosis, and a basolateral domain, which senses variations of the internal milieu and is enriched with receptors for various hormones and secretagogues. It is unknown whether secretion is polarized in endocrine cells (except for thyroid follicular cells, which are organized into cavitary structures). To determine whether distinct plasma membrane domains exist in endocrine cells, we infected monolayer cultures of pancreatic endocrine cells with enveloped RNA viruses known to bud selectively from either the apical or basolateral domain in polarized epithelial cells1. This asymmetrical budding is thought to reflect the polarized nature of the infected cells, as in non-polarized cells such as fibroblasts, the same viruses bud nonselectively from the entire cell surface1. We show here that influenza virus and vesicular stomatitis virus (VSV) emerge asymmetrically from cultured pancreatic islet cells; this represents the first evidence for polarization of plasma membrane domains in pancreatic endocrine cells.

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Authors and Affiliations

  1. Institute of Histology and Embryology, University of Geneva Medical School, 1211, Geneva, 4, Switzerland
    Tommaso Lombardi, Roberto Montesano, Annelise Wohlwend, Mylène Amherdt, Jean-Dominique Vassalli & Lelio Orci

Authors

  1. Tommaso Lombardi
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  2. Roberto Montesano
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  3. Annelise Wohlwend
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  4. Mylène Amherdt
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  5. Jean-Dominique Vassalli
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  6. Lelio Orci
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Lombardi, T., Montesano, R., Wohlwend, A. et al. Evidence for polarization of plasma membrane domains in pancreatic endocrine cells.Nature 313, 694–696 (1985). https://doi.org/10.1038/313694a0

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