Identical Vβ T-cell receptor genes used in alloreactive cytotoxic and antigen plus I–A specific helper T cells (original) (raw)

Nature volume 315, pages 425–427 (1985)Cite this article

Abstract

T lymphocytes involved in the cellular immune response carry cell-surface receptors, responsible for antigen and self recognition. This T-cell receptor molecule is a heterodimeric protein consisting of disulphide-linked _α_- and _β_-chains with variable (V) and constant (C) regions1–5. Several complementary DNA and genomic DNA clones have been isolated and characterized6–12. These analyses showed that the genomic arrangement and rearrangement of T-cell receptor genes using _V_T, diversity (_D_T), joining (_J_T) and _C_T gene segments is very similar to the structure of the known immunoglobulin genes (see ref. 13 for review). We have isolated two cDNA clones from an allospecific cytotoxic T cell, one of which shows a productive V β_–_J β_–_C β 1 rearrangement without an intervening _D_β segment. This V β gene segment is identical to the V β gene expressed in a helper T-cell clone specific for chicken red blood cells and H–2Ib (ref. 14). The other clone carries the C _β_2 gene of the T-cell receptor, but the C _β_2 sequence is preceded by a DNA sequence that does not show any similarity to V β or J β sequences.

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Authors and Affiliations

  1. Abteilung für Experimentelle Pathologie, Universitätsspital, Sternwartstrasse 2, Zürich, 8091, Switzerland
    Fabio Rupp, Hans Acha-Orbea, Hans Hengartner, Rolf Zinkernagel & Rolf Joho

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  1. Fabio Rupp
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  2. Hans Acha-Orbea
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  3. Hans Hengartner
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  4. Rolf Zinkernagel
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  5. Rolf Joho
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Rupp, F., Acha-Orbea, H., Hengartner, H. et al. Identical V β T-cell receptor genes used in alloreactive cytotoxic and antigen plus I–A specific helper T cells.Nature 315, 425–427 (1985). https://doi.org/10.1038/315425a0

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