Control of transcription of α-amylase and rRNA genes in barley aleurone protoplasts by gibberellin and abscisic acid (original) (raw)

Nature volume 316, pages 275–277 (1985) Cite this article

Abstract

Gibberellin A3 (GA3) promotes and abscisic acid (ABA) inhibits the production of _α_-amylase in aleurone layers of barley (Hordeum vulgare L.); this sytem has been used extensively to study the mechanisms of action of these hormones1. Cell-free messenger RNA translation studies have shown that the GA3-promoted increase in _α_-amylase synthesis and its inhibition by ABA are associated with corresponding changes in the levels of translatable _α_-amylase mRNA2–5. Subsequent studies using hybridization of an _α_-amylase complementary DNA-rich probe6 and of clonedα-amylase cDNA probe6 to aleurone RNA7–9 have shown that the abundance of _α_-amylase mRNA increases in response to GA3, and that this increase is inhibited by ABA. The increased abundance of mRNA could result from an increased rate of transcription, increased stability of mRNA, or both, as in some animal cells10–12. By using methods for studying gene transcription in isolated plant nuclei13–15, we present here evidence that GA3 and ABA regulate the transcription of both _α_-amylase and ribosomal RNA (rRNA) genes

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References

  1. Jacobsen, J. V. in The Biochemistry and Physiology of Gibberellins Vol. 2 (ed. Crozier, A.) 159–187 (Praeger, 1983).
    Google Scholar
  2. Higgins, T. J. V., Zwar, J. A. & Jacobsen, J. V. Nature 260, 166–169 (1976).
    Article ADS CAS Google Scholar
  3. Mozer, T. Cell 20, 479–485 (1980).
    Article CAS Google Scholar
  4. Muthukrishnan, S., Chandra, G. R. & Maxwell, E. S. Proc. natn. Acad. Sci. U.S.A. 76, 6181–6185 (1979).
    Article ADS CAS Google Scholar
  5. Higgins, T. J. V., Jacobsen, J. V. & Zwar, J. A. Pl. molec. Biol. 1, 191–215 (1982).
    Article CAS Google Scholar
  6. Bernal-Lugo, I., Beachy, R. N. & Varner, J. E. Biochem. biophys. Res. Commun. 102, 617–623 (1981).
    Article CAS Google Scholar
  7. Muthukrishnan, S., Chandra, G. R. & Maxwell, E. S. J. biol. Chem. 258, 2370–2375 (1983).
    CAS PubMed Google Scholar
  8. Rogers, J. C. & Milliman, C. J. Biol. Chem. 258, 8169–8174 (1983).
    CAS Google Scholar
  9. Chandler, P. M., Zwar, J. A., Jacobsen, J. V., Higgins, T. J. V. & Inglis, A. S. Pl. molec. Biol. 3, 407–418 (1984).
    Article CAS Google Scholar
  10. McKnight, G. S. & Palmiter, R. D. J. biol. Chem. 254, 9050–9058 (1979).
    CAS PubMed Google Scholar
  11. Wiskocil, R. et al. Proc. natn. Acad. Sci. U.S.A. 77, 4474–4478 (1980).
    Article ADS CAS Google Scholar
  12. Brock, M. L. & Shapiro, D. J. Cel 34, 207–214 (1983).
    Article CAS Google Scholar
  13. Gallagher, T. F. & Ellis, R. J. EMBO J. 1, 1493–1498 (1982).
    Article CAS Google Scholar
  14. Evans, I. M., Gatehouse, J. A., Cray, R. R. D. & Boulter, D. Planta 160, 559–568 (1984).
    Article CAS Google Scholar
  15. Chappell, J. & Hahlbrock, K. Nature 311, 76–78 (1984).
    Article ADS CAS Google Scholar
  16. Jacobsen, J. V., Zwar, J. A. & Chandler, P. M. Planta 163, 430–439 (1985).
    Article CAS Google Scholar
  17. Beach, L. R., Spencer, D., Randall, P. J. & Higgins, T. J. V. Nucleic Acids Res. 13, 999–1013 (1985).
    Article CAS Google Scholar
  18. Baulcombe, D. C. & Buffard, D. Planta 157 493–501 (1983).
    Article CAS Google Scholar
  19. Luthe, D. S. & Quatrano, R. S. Pl. Physiol. 65, 305–308 (1980).
    Article CAS Google Scholar
  20. Gerlach, W. L. & Bedbrook, J. R. Nucleic Acids Res. 7, 1869–1885 (1979).
    Article CAS Google Scholar
  21. Chandler, P. M., Spencer, D., Randall, P. J. & Higgins, T. J. V. Pl. Physiol. 75, 651–657 (1984).
    Article CAS Google Scholar
  22. Thomas, P. S. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1980).
    Article ADS CAS Google Scholar

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Author notes

  1. L. R. Beach
    Present address: Pioneer Hi-Bred International Inc., Plant Breeding Division, Department of Biotechnology Research, 7300 N-W 62 Avenue, Box 38, Johnston, Iowa, 50134, USA

Authors and Affiliations

  1. Division of Plant Industry, CSIRO, PO Box 1600, Canberra, ACT, 2601, Australia
    J. V. Jacobsen & L. R. Beach

Authors

  1. J. V. Jacobsen
  2. L. R. Beach

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Jacobsen, J., Beach, L. Control of transcription of _α_-amylase and rRNA genes in barley aleurone protoplasts by gibberellin and abscisic acid.Nature 316, 275–277 (1985). https://doi.org/10.1038/316275a0

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