Neuronal cell–cell adhesion depends on interactions of N-CAM with heparin-like molecules (original) (raw)
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- Published: 03 April 1986
Nature volume 320, pages 445–447 (1986)Cite this article
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Abstract
Cell–cell interactions are of critical importance during neural development, particularly since the migration of neural cells and the establishment of functional interactions between growing axons and their target cells1,2 has been suggested to depend upon cell recognition processes. Neurone–neurone adhesion has been well studied in vitro, and is mediated in part by the neural cell adhesion molecule N-CAM3–7. N-CAM-mediated cell–cell adhesion has been postulated to occur by a homophilic binding mechanism8, in which N-CAM on the surface of one cell binds to N-CAM on a neighbouring cell. Studies in our laboratory have identified a cell surface glycoprotein, now known to be N-CAM9, which participates in cell–substratum interactions in the developing chicken nervous system10–12. Although this adhesion involves a homophilic binding mechanism, the binding of the cell surface proteoglycan heparan sulphate to the glycoprotein is also required13. This raises the question of whether the binding of heparan sulphate to N-CAM is also required for cell-cell adhesion. Here we show that the binding of retinal probe cells to retinal cell monolayers is inhibited by heparin, a functional analogue of heparan sulphate, but not by chondroitin sulphate. Monoclonal antibodies that recognize two different domains on N-CAM, the homophilic-binding and heparin-binding domains, inhibit cell–cell adhesion. The heparin-binding domain isolated from N-CAM by selective proteolysis also inhibits cell-cell adhesion when bound to the probe cells.
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References
- Rakic, P. & Sidman, R. L. J. comp. Neurol. 152, 103–161 (1973).
Article CAS Google Scholar - Rutishauser, U., Grumet, M. & Edelman, G. M. J. Cell Biol. 97, 145–152 (1983).
Article CAS Google Scholar - Thiery, J. P., Brackenbury, R., Rutishauser, U. & Edelman, G. M. J. biol. Chem. 252, 6841–6845 (1977).
CAS Google Scholar - Jorgensen, O. S., Delouvee, A., Thiery, J. P. & Edelman, G. M. FEES Lett. 111, 39–42 (1980).
Article CAS Google Scholar - Hirn, M., Ghandour, M. S., Deagostini-Bazin, H. & Goridis, C. Brain Res. 265, 87–100 (1983).
Article CAS Google Scholar - Edelman, G. M. Science 219, 450–457 (1983).
Article ADS CAS Google Scholar - Rutishauser, U. Nature 310, 549–554 (1984).
Article ADS CAS Google Scholar - Rutishauser, U., Hoffman, S. & Edelman, G. M. Proc. natn. Acad. Sci. U.S.A. 79, 685–689 (1982).
Article ADS CAS Google Scholar - Cole, G. J. & Glaser, L. J. Cell Biol. 102, 403–412 (1986).
Article CAS Google Scholar - Cole, G. J. & Glaser, L. Proc. natn. Acad. Sci. U.S.A. 81, 2260–2264 (1984).
Article ADS CAS Google Scholar - Cole, G. J. & Glaser, L. J. biol. Chem. 259, 4031–4034 (1984).
CAS PubMed Google Scholar - Cole, G. J. & Glaser, L. J. Cell Biol. 99, 1605–1612 (1984).
Article CAS Google Scholar - Cole, G. J., Schubert, D. & Glaser, L. J. Cell Biol. 100, 1192–1199 ((1985).
Article CAS Google Scholar - Gottlieb, D. I., Rock, K. & Glaser, L. Proc. natn. Acad. Sci. U.S.A. 73, 410–414 (1976).
Article ADS CAS Google Scholar - Schubert, D. & LaCorbiere, M. J. Cell Biol. 100, 56–63 (1985).
Article CAS Google Scholar - Edgar, D., Timpl, R. & Thoenen, H. EMBO J. 3, 1463–1468 (1984).
Article CAS Google Scholar - Rogers, S. L., McCarthy, J. B., Palm, S. L., Furcht, L. T. & Letourneau, P. C. J. Neurosci. 5, 369–378 (1985).
Article CAS Google Scholar
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Authors and Affiliations
- Department of Biological Chemistry, Division of Biology and Biomedical Sciences, Washington University School of Medicine, 660 S. Euclid Avenue, St Louis, Missouri, 63110, USA
Gregory J. Cole, Arleen Loewy & Luis Glaser
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- Gregory J. Cole
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Cole, G., Loewy, A. & Glaser, L. Neuronal cell–cell adhesion depends on interactions of N-CAM with heparin-like molecules.Nature 320, 445–447 (1986). https://doi.org/10.1038/320445a0
- Received: 30 October 1985
- Accepted: 13 February 1986
- Issue Date: 03 April 1986
- DOI: https://doi.org/10.1038/320445a0