Fluorescence detection in automated DNA sequence analysis (original) (raw)
- Article
- Published: 12 June 1986
- Jane Z. Sanders1,
- Robert J. Kaiser1,
- Peter Hughes1,
- Chris Dodd1,
- Charles R. Connell1 na1,
- Cheryl Heiner1 na1,
- Stephen B. H. Kent1 &
- …
- Leroy E. Hood1
Nature volume 321, pages 674–679 (1986)Cite this article
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Abstract
We have developed a method for the partial automation of DNA sequence analysis. Fluorescence detection of the DNA fragments is accomplished by means of a fluorophore covalently attached to the oligonucleotide primer used in enzymatic DNA sequence analysis. A different coloured fluorophore is used for each of the reactions specific for the bases A, C, G and T. The reaction mixtures are combined and co-electrophoresed down a single polyacrylamide gel tube, the separated fluorescent bands of DNA are detected near the bottom of the tube, and the sequence information is acquired directly by computer.
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Author notes
- Charles R. Connell and Cheryl Heiner: Applied Biosystems, Inc., Foster City, California 94404, USA
Authors and Affiliations
- Division of Biology, California Institute of Technology, Pasadena, California, 91125, USA
Lloyd M. Smith, Jane Z. Sanders, Robert J. Kaiser, Peter Hughes, Chris Dodd, Charles R. Connell, Cheryl Heiner, Stephen B. H. Kent & Leroy E. Hood
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- Lloyd M. Smith
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Cite this article
Smith, L., Sanders, J., Kaiser, R. et al. Fluorescence detection in automated DNA sequence analysis.Nature 321, 674–679 (1986). https://doi.org/10.1038/321674a0
- Received: 30 December 1985
- Accepted: 11 April 1986
- Issue Date: 12 June 1986
- DOI: https://doi.org/10.1038/321674a0